Publications Dr. Katharina Wenzel-Seifert

Doctoral Thesis (M.D.):

Review Articles

Klinker J F, Wenzel-Seifert K , Seifert R (1996) G-Protein-coupled receptors in HL-60 human leukemia cells. Gen Pharmac 27:33-54

Seifert R, Wenzel-Seifert K and Kobilka BK (1999) GPCR- G a fusion proteins: molecular analysis of receptor-G-protein coupling. Trends Pharmacol Sci 20: 383-389

Seifert R and Wenzel-Seifert K (2002) Constitutive activity of G-protein-coupled receptors: cause of disease and common property of wild-type receptors. Naunyn-Schmiedebergs Arch : 366:381-416

Monographs and Book Chapters

Wenzel-Seifert K, Seifert R (2001) Molecular mechanisms of chemoattractant receptor-G-protein coupling. In: Physiology of Inflammation (Ley, K., ed.). Oxford University Press, New York, pp. 146-189

Seifert R., Wenzel-Seifert K. (2002) Use of GPCR-Gα fusion proteins for the analysis of GPCR/G-protein coupling. In: Gene Clonin & Expression Technologies (Weiner M, Liu Q, eds.). Eaton, Natick, pp 305-326

Clinical Paper

Wenzel-Seifert K, Sharma AM, Keller F (1990) Repeated dialysis anaphylaxia. Nephrol Dial Transplant 5: 821-824

Wenzel-Seifert K, Harwig S, Keller F (1991) Fulminant systemic calcinosis in two patients after kidney transplantation. Am J Nephrol 11: 497-500

Research paper

Seifert R, Wenzel K, Eckstein F, Schultz G (1989) Purine and pyrimidine nucleotidespotentiate activation of NADPH oxidase and degranulation by chemotactic peptides andinduce aggregation of human neutrophils via G proteins. Eur J Biochem 181: 277-285

Wenzel-Seifert K, Seifert R (1990) Nucleotide-, chemotactic peptide- and phorbol ester-induced exocytosis in HL-60 leukemic cells. Immunobiology 181: 298-315

Wenzel-Seifert K, Ervens J, Seifert R (1991) Differential inhibition and potentiation by cell permeant analogues of cyclic AMP and cyclic GMP and NO-containing compounds of exocytosis in human neutrophils. Naunyn-Schmiedeberg's Arch Pharmacol 344: 396-402

Wenzel-Seifert K, Grünbaum L, Seifert R (1991) Differential inhibition of human neutrophil activation by cyclosporines A, D and H: Cyclosporin H is a potent and effective inhibitor of formyl peptide-induced superoxide formation. J Immunol 147: 1940-1946

Wenzel-Seifert K, Seifert, R (1993) Cyclosporin H is a potent and selective formyl peptide receptor antagonist: Comparison with N-t-butoxycarbonyl-L-phenylalanyl-L-leucyl-L-phenylalanyl-L-leucyl-L-phenyl-alanine and the cyclosporins A, B, C, D and E. J Immunol 150: 4591-4599

Wenzel-Seifert K, Seifert, R (1993) Partial inhibition of human neutrophil activation by FK-506 at supratherapeutic concentrations. Naunyn-Schmiedeberg's Arch Pharmacol 348: 7-13

Klinker JF, Höer A, Schwaner I, Offermanns S, Wenzel-Seifert K, Seifert R (1993) Lipopeptides activate G_i-proteins in dibutyryl cAMP-differentiated HL-60 cells. Biochem J 296: 245-251

Wagner S, Wenzel-Seifert K, Volbracht L, Sorgenfrei D, Ebel H (1994). Oleic acid inhibition of Na⁺-D-glucose transport in isolated renal brush border membranes. Role of lipid physical parameters and trans Na⁺inhibition. Biochem Biophys Acta 1190: 309-318

Wenzel-Seifert K, Schächtele C, Seifert R (1994) N-Protein kinase C isoenzymes may be involved in the regulation of various neutrophil functions. Biochem Biophys Res Comm 200: 1536-1543

Klinker JF, Hagelüken A, Grünbaum L, Nürnberg B, Harhammer R, Offermanns S, Schwaner I, Ervens J, Wenzel-Seifert K , Müller T, Seifert R (1994) Mastoparan may activate GTP hydrolysis by G_i-proteins in HL-60 membranes indirectly through interaction with nucleoside diphosphate kinase. Biochem J 304: 377-383

Wenzel-Seifert K, Schächtele C, Hummel R, Grünbaum L, Seifert R (1994) Evidence that inhibition of phorbol ester-induced superoxide anion formation by Cyclosporin A in phagocytes is not mediated by direct inhibition of protein kinase C. Biochem Pharmacol 48: 859-864

Wenzel-Seifert K, Krautwurst D, Musgrave I, Seifert R. (1996) Thapsigargin activates univalent- and bivalent cation entry in human neutrophils by a SK&F 96365- and Gd³⁺-sensitive pathway and is a partial secretagogue: Involvement of pertussis-toxin-sensitive G-proteins and protein phosphatases 1/2A and 2B in the signal-transduction pathway. Biochem J 314: 679-686

Wenzel-Seifert K, Krautwurst D, Lentzen H, Seifert R. (1996) Concanavalin A and mistletoe lectin I differentially activate cation entry and exocytosis in human neutrophils: Lectins may activate multiple subtypes of cation channels. J Leukocyte Biol 60: 345-355

Wenzel-Seifert K, Lentzen H, Seifert R. (1997) In U-937 promonocytes, misteltoe lectin I increases basal [Ca²⁺]_i, enhances histamine H₁- and complement C5a-receptor-mediated rises in [Ca²⁺]_i, and induces cell death. Naunyn-Schmiedeberg's Arch Pharmacol 355: 190-197

Wenzel-Seifert K, Lentzen H, Aktories K, Seifert R (1997) Complex regulation of human neutrophil activation by actin filaments: Dihydrocytochalasin B and botulinum C2 toxin uncover the existence of multiple cation entry pathways. J Leukocyte Biol 61: 703-711

Seifert R, Wenzel-Seifert K, Lee TW, Gether U, Sanders-Bush E, Kobilka BJ (1998) Different effects of G_sα splice variants on β₂-adrenoreceptor-mediated signaling. The β₂-adrenoceptor coupled to the long splice variant of G_sα has properties of a constitutively active receptor. J Biol Chem 273: 5109-5116

Wenzel-Seifert K, Hurt CM and Seifert R (1998) High constitutive activity of the human formyl peptide receptor. J Biol Chem 77: 24181-24189

Wenzel-Seifert K, Lee TW, Seifert R and Kobilka BK (1998) Restricting mobility of G_sα relative to the β₂-adrenoceptor enhances adenylate cyclase activity by reducing G_sα GTPase activity. Biochem J 334: 519-524

Seifert R, Gether U, Wenzel-Seifert K and Kobilka BK (1999) Effects of guanine, inosine, and xanthine nucleotides on β-adrenergic receptor/G_sα interactions: Evidence for multiple receptor confirmations. Mol Pharmacol 56: 348-358

Seifert R, Wenzel-Seifert K, Gether U, Lam VT and Kobilka BK (1999) Examining the efficiency of receptor/G-protein coupling with a cleavable β₂-adrenoceptor-G_sα fusion protein. Eur J Biochem 260: 661-666

Wenzel-Seifert K, Arthur JM, Liu HY and Seifert R (1999) Quantitative analysis of formyl peptide receptor coupling to G_iα₁, G_iα₂, and G_iα₃. J Biol Chem 274: 33259-33266.

Seifert, R., Wenzel-Seifert , K., Kobilka, B. K. (2000) G-protein-coupled receptors - what limits high-affinity agonist binding? Reply to letter of Dr. S. Hoare. Trends Pharmacol Sci. 21: 83-84.

Wenzel-Seifert K, Seifert R (2000) Molecular analysis of β₂-adrenoceptor coupling to G_s-, G_i- and G_q- proteins. Mol Pharmacol 58: 954-966.

Wenzel-Seifert, K. and Seifert, R. (2001): Defective G_i-protein coupling in two formyl peptide receptor mutants associated with juvenile periodontitis. J. Biol. Chem. 276: 42043-42049.

Wenzel-Seifert, K. , Kelley, M. T., Buschauer, A. and Seifert, R. (2001): Similar apparent constitutive activity of the human histamine H₂-receptor fused to the long and short splice variants of G_sα . J. Pharmacol. Exp. Ther. 299: 1013-1020

Kelley, M. T., Bürckstümmer, T., Wenzel-Seifert, K., Dove, S., Buschauer, A. and Seifert, R. (2001): Distinct interaction of human and guinea pig histamine H₂-receptor with guanidine-type agonists. Mol. Pharmacol. 60: 1210-1225.

Liu, H.-Y., Wenzel-Seifert, K. and Seifert, R. (2001): The olfactory G-protein G_olfα possesses a lower GDP-affinity and deactivates more rapidly than G_sαshort: Consequences for receptor-coupling and adenylyl cyclase activation. J. Neurochem. 78:325-338 .

Seifert, R. and Wenzel-Seifert, K. (2001) Unmasking different constitutive activity of four chemoattractant receptors using Na⁺ as universal stabilizer of the inactive (R) state. Receptors Channels 7: 357-369.

Seifert, R., Wenzel-Seifert, K., Gether, U., Kobilka, B. K. (2001) Functional differences between full and partial agonists: Evidence for ligand-specific receptor conformations. J. Pharmacol Exp Ther 297:1218-1226.

Houston, C., Wenzel-Seifert, K., Bürckstümmer, T. and Seifert, R. (2002): The human histamine H₂ receptor couples more efficiently to Sf9 insect cell G_s-proteins than to insect cell G_q-proteins: Limitations of Sf9 cells for the analysis of receptor/G_q-protein. J. Neurochem. 80: 678-696.

Wenzel-Seifert, K., Liu, H.-Y. and Seifert, R. (2002): Similarities and differences in the coupling of human β₁- and β₂-adrenoceptor to G_sα splice variants. Biochem. Pharmacol 64: 9-20.http://www.ipt.med.tu-muenchen.de/For/FOR923.htm

Seifert R, Wenzel-Seifert K, Arthur JM, Jose PO and Kobilka BK (2002) Efficient adenylyl cyclase by a β₂-adrenoceptor-Gi_α2 fusion protein. Biochem Biophys Res Commun 298: 824-828.

Gille A, Wenzel-Seifert K, Doughty MB and Seifert R (2003) GDP-affinity and order of the catalytic site are critical for function of xanthine nucleotide-selective G_α proteins. J Biol Chem 278: 7822-7828.

Wenzel-Seifert K and Seifert R (2003) Properties of Arg389-β₁-adrenoceptor-G_sα fusion proteins. Receptor Channels 9; 315-323.

Seifert R, Wenzel-Seifert K, Bürckstümmer T, Pertz HH, Schunack W, Dove S, Buschauer A, Elz S (2003) Multiple differences in agonist and antagonist pharmacology between human and guinea pig histamine H₁-receptor. J Pharmacol Exp Ther 305: 1104-1115.

Wenzel-Seifert K, Seifert R (2003) Critical role of N-terminal N-glycosylation for proper folding of the human formyl peptide receptor. Biochem Biophys Res Commun 301: 693-698.