Determination of drugs and new compounds

• Quantitation of cytostatics from biological material by HPLC, CE and AAS
  (e.g. melphalan, gemcitabin, vinblastin, vincristin, paclitaxel, methotrexat, platinum complexes, anthracyclines, dacarbazin)
• HPLC and CE methods for the determination of guanidine-type histamine H₂ agonists, argininamide-type NPY antagonists, corresponding prodrugs and synthetic intermediates
• Analysis of endogenous and aministered hyaluronidase in humans and experimental animals

Selected publications:

• G. Bernhardt, M. Koch, T. Spruß, R. Gust, R. Krauser, R. Schlemmer, M. Hollstein, F. Lux, H. Schönenberger, [meso-1,2-Bis(2,6-dichloro-4-hydroxyphenyl)ethylenediamine]sulfatoplatinum(II) - Pharmacokinetic Studies. Arch. Pharm. Pharm. Med. Chem. 332, 195-200 (1999).
• I. Muckenschnabel, G. Bernhardt, T. Spruß, A. Buschauer, Hyaluronidase pretreatment produces selective melphalan enrichment in malignant melanoma implanted in nude mice. Cancer Chemother. Pharmacol. 38, 88-94 (1996).
• I. Muckenschnabel, G. Bernhardt, T. Spruß, B. Dietl, A. Buschauer. Quantitation of hyaluronidases by the Morgan-Elson reaction: comparison of the enzyme activities in the plasma of tumor patients and healthy volunteers. Cancer Lett. 131, 13-20 (1998).
• I. Muckenschnabel, G. Bernhardt, T. Spruß, A. Buschauer, Pharmacokinetics and tissue distribution of bovine testicular hyaluronidase and vinblastine in mice: an attempt to optimize the mode of adjuvant hyaluronidase administration in cancer chemotherapy. Cancer Lett. 131, 71-84 (1998).
• A. Schuster, G. Bernhardt, A. Buschauer, Determination of the arpromidine-type histamine H₂-receptor agonist N¹-[3-(3,4-difluorophenyl)-3-(2-pyridyl)propyl]-N²-[3-(1H-imidazol-4-yl)propyl]guanidine and corresponding N³-alkoxycarbonylguanidines by HPLC and CE. Eur. J. Pharm. Sci. 5, 79-88 (1997).

• Th. Weiss, G. Bernhardt, A. Buschauer, K. W. Jauch, H. Zirngibl, High-resolution RP HPLC analysis of polyamines and their monoacetyl conjugates by fluorescence detection after derivatization with N-hydroxy-succinimidyl 6-quinolinyl carbamate. Anal. Biochem. 247, 294-304 (1997).
• I. Muckenschnabel, G. Bernhardt, Th. Spruß, A. Buschauer, A versatile HPLC method for the measurement of melphalan tailored to the optimization of hyperthermic isolated limb perfusion. Eur. J. Pharm. Sci. 5, 129-137 (1997).