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Publications


Fakultät für Biologie und Vorklinische Medizin
Institute of Plant Sciences
Cell Biology and Plant Biochemistry
Prof. Dr. Klaus Grasser - Publications

Publications


2017
 
Antosz, W., Pfab, A., Ehrnsberger, H.F., Holzinger, P., Köllen, K., Mortensen, S.A., Bruckmann, A., Schubert, T., Längst, G., Griesenbeck, J., Schubert, V., Grasser, M. and Grasser, K.D. (2017) Composition of the Arabidopsis RNA polymerase II transcript elongation complex reveals the interplay between elongation and mRNA processsing factors. Plant Cell 29, 854-870.

Sørensen, B.B., Ehrnsberger, H.F., Esposito, S., Pfab, A., Bruckmann, A., Hauptmann, A., Meister, G., Merkl, R., Schubert, T., Längst, G., Melzer, M., Grasser, M. and Grasser, K.D. (2017) The Arabidopsis THO/TREX component TEX1 functionally interacts with MOS11 and modulates mRNA export and alternative splicing events. Plant Mol. Biol. 93, 283-298.

Pfab, A., Antosz, W., Holzinger, P., Bruckmann, A., Griesenbeck, J. and Grasser, K.D. (2017) Analysis of in vivo chromatin and protein interactions of Arabidopsis transcript elongation factors. Meth. Mol. Biol. 1629, 105-122.


2015
 
Antosch, M., Schubert, V., Holzinger, P., Houben, A. and Grasser, K.D. (2015) Mitotic lifecycle of chromosomal 3xHMG-box proteins and role of their N-terminal domain in the association with rDNA loci and proteolysis. New Phytol. 208, 1067-1077.

Köllen, K., Dietz, L., Bies-Eteve, N., Lagrange, T., Grasser, M. and Grasser, K.D. (2015) The zinc-finger protein SPT4 interacts with SPT5L/KTF1 and modulates transcriptional silencing in Arabidopsis. FEBS Lett. 589, 3254-3257.

 

2014

Van Lijsebettens, M. and Grasser, K.D. (2014) Transcript elongation factors: shaping transcriptomes after transcript initiation. Trends Plant Sci. 19, 717-726.

Stott, K., Watson, M., Bostock, M.J., Mortensen, S.A., Travers, A., Grasser, K.D. and Thomas, J.O. (2014) Structural insights into the mechanism of negative regulation of single HMG-box proteins by the acidic tail domain. J. Biol. Chem. 289, 29817-29826.

Van Lijsebettens, M., Dürr, J., Woloszynska, M. and Grasser, K.D. (2014) Elongator and SPT4/SPT5 complexes as proxy to study RNA polymerase II transcript elongation control of plant development. Proteomics 14, 2109-2114.

Dürr, J., Lolas, I.B., Sørensen, B.B., Schubert,V., Houben, A., Melzer, M., Deutzmann, R., Grasser, M. and Grasser, K.D. (2014) The transcript elongation factor SPT4/SPT5 is involved in auxin-related gene expression in Arabidopsis. Nucleic Acids Res. 42, 4332-4347.

Mortensen, S.A. and Grasser, K.D. (2014) The seed dormancy defect of Arabidopsis mutants lacking the transcript elongation factor TFIIS is caused by reduced expression of the DOG1 gene. FEBS Lett. 588, 47-51.


2013

Kammel, C,. Thomaier, M., Sørensen B.B., Schubert, T., Längst, G., Grasser, M. and Grasser, K.D. (2013) Arabidopsis DEAD-Box RNA helicase UAP56 interacts with both RNA and DNA as well as with mRNA export factors. PLOS ONE 8, e60644.



2012

Antosch, M., Mortensen, S.A. and Grasser, K.D. (2012) Plant proteins containing HMG-box DNA-binding domains modulate different nuclear processes. Plant Physiol. 159, 875-883.



2011

Merkle, T. and Grasser, K.D. (2011) Unexpected mobility of plant chromatin-associated HMGB proteins. Plant Signal. Behav. 6, 878-880.

Pedersen, D.S., Coppens, F., Ma, L., Antosch, M., Marktl, B., Merkle, T., Beemster, G.T.S., Houben, A. and Grasser, K.D. (2011) The plant-specific family of DNA-binding proteins containing three HMG-box domains interacts with mitotic and meiotic chromosomes. New Phytol.192, 577-589.

Mortensen, S.A., Sønderkær, M., Lynggaard, C., Grasser, M., Nielsen, K.L. and Grasser, K.D. (2011) Reduced expression of the DOG1 gene in Arabidopsis mutant seeds lacking the transcript elongation factor TFIIS. FEBS Lett. 585, 1929-1933.

Finnie, C., Sultan, A. and Grasser, K.D. (2011) From protein catalogues towards targeted proteomics approaches in cereal grains. Phytochemistry 72, 1145-1153.

Schrumpfová, P.P., Fojtová,  M., Mokroš, P., Grasser, K.D. and Fajkus, J. (2011) Role of HMGB proteins in chromatin dynamics and telomere maintenance in Arabidopsis thaliana. Curr. Protein Pept. Sci. 12, 105-111.



2010

Van Lijsebettens, M. and Grasser, K.D. (2010) The role of the transcript elongation factors FACT and HUB1 in leaf growth and the induction of flowering. Plant Signal. Behav. 5, 715-717.

Pedersen,D.S., Merkle,T., Marktl,B., Lildballe,D.L., Antosch,M., Bergmann,T., Tönsing,K., Ansemetti,D., and Grasser,K.D. (2010). Nucleocytoplasmic distribution of the Arabidopsis chromatin-associated HMGB2/3 and HMGB4 proteins. Plant Physiol. 154, 1831-1841.

Kalamajka, R., Finnie, C. and Grasser, K.D. (2010) Onset of grain filling is associated with a change in properties of linker histone variants in maize kernels. Planta 231, 1127-1135.

Lolas, I.B., Himanen, K., Grønlund, J.T., Lynggaard, C., Houben, A., Melzer, M., Van Lijesebettens, M. and Grasser, K.D. (2010) The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2. Plant J., 61, 686-697.

Pedersen, D.S. and Grasser, K.D. (2010) The role of chromosomal HMGB proteins in plants. Biochim. Biophys. Acta 1799, 171-174.



2009

Grasser, M., Kane, C.M., Merkle, T., Melzer, M., Emmersen, J. and Grasser, K.D. (2009) Transcript elongation factor TFIIS is involved in Arabidopsis seed dormancy. J. Mol. Biol. 386, 598-611.

 

2008

Hansen, F.T., Madsen, C.K., Nordland, A.M., Grasser, M., Merkle, T. and Grasser, K.D. (2008) A novel family of plant DNA-binding proteins containing both HMG-box and AT-rich interaction domains. Biochemistry 47, 13207-13214.

Launholt, D., Pedersen, D.S., Kalamajka, R., Emmersen, J., Houben, A. and Grasser, K.D. (2008) The expression level of the chromatin-associated HMGB1 protein influences growth, stress tolerance and transcriptome in Arabidopsis. J. Mol. Biol. 384, 9-21.

Lyngaard, C., Stemmer, C., Stensballe, A., Graf, M., Gorr, G., Decker, E. and Grasser, K.D. (2008) Physcomitrella HMGA-type proteins display structural differences compared to their higher plant counterparts. Biochem. Biophys. Res. Commun. 374, 653-657.

Qiu, J.-L., Fiil, B.K., Petersen, K., Nielsen, H.B., Botanga, C.J., Thorgrimsen, S., Palma, K., Suarez-Rodriguez, M.C., Sandbech-Clausen, S., Lichota, J., Brodersen,P., Grasser, K.D., Mattsson, O., Glazebrook, J., Mundy, J. and Petersen, M. (2008) Arabidopsis MAP kinase 4 regulates gene expression via transcription factor release in the nucleus. EMBO J. 27, 2214-2221.
 
Kiilerich, B., Stemmer, C., Merkle, T., Launholt, D., Gorr, G. and Grasser, K.D. (2008) Chromosomal high mobility group (HMG) proteins of the HMGB-type occurring in the moss Physcomitrella patens. Gene 407, 86-97.

 

2007

Stros, M., Launholt, D. and Grasser, K.D. (2007) The HMG-box: a versatile protein domain occurring in a wide variety of DNA-binding proteins. Cell. Mol. Life Sci. 64, 2590-2606.

Grasser, M., Christensen, J.M., Peterhänsel, C. and Grasser, K.D. (2007) Basic and acidic regions flanking the HMG-box domain of maize HMGB1 and HMGB5 modulate the stimulatory effect on the DNA binding of transcription factor Dof2. Biochemistry 46, 6375-6382.

Grasser, K.D., Launholt, D. and Grasser, M. (2007) High mobility group proteins of the plant HMGB family: dynamic chromatin modulators. Biochim. Biophys. Acta 1769, 346-357.

Launholt, D., Grønlund, J.T, Nielsen, H.K. and Grasser, K.D. (2007) Overlapping expression patterns among the genes encoding Arabidopsis chromosomal high mobility group (HMG) proteins. FEBS Lett. 581, 1114-1118.

Grønlund, J.T, Stemmer, C., Lichota, J., Merkle, T.  and Grasser, K.D. (2007) Functionality of the β/six site-specific recombination system in tobacco and Arabidopsis: a novel tool for genetic engineering of plant genomes. Plant Mol. Biol. 63, 545-556.

 

2006

Launholt, D., Merkle, T., Houben, A., Schulz, A. and Grasser, K.D. (2006) Arabidopsis chromatin-associated HMGA and HMGB proteins use different nuclear targeting signals and display a highly dynamic localization within the cell nucleus. Plant Cell 18, 2904-2918.

Grasser, M., Lentz, A., Lichota, J., Merkle, T. and Grasser, K.D. (2006) The Arabidopsis genome encodes structurally and functionally diverse HMGB-type proteins. J. Mol. Biol. 358, 654-664.

 

2005

Grasser, K.D. (2005) Emerging role for transcript elongation in plant development. Trends Plant Sci. 10, 484-490.

 

2004

Duroux, M., Houben, A., Ruzicka, K., Friml, J. and Grasser, K.D. (2004). The chromatin remodelling complex FACT associates with actively transcribed regions of the Arabidopsis genome. Plant J. 40, 660-671.

Thomsen, M.S., Franßen, L., Launholt, D., Fojan, P. and Grasser, K.D. (2004) Interactions of the basic N-terminal and the acidic C-terminal domains of the maize chromosomal HMGB1 protein. Biochemistry 43, 8029-8037.

Lichota, J., Ritt, C. and Grasser, K.D. (2004) Ectopic expression of the maize chromosomal HMGB1 protein causes defects in root development in tobacco seedlings. Biochem. Biophys. Res. Commun. 318, 317-322.

Grasser, K.D., Grill, S., Duroux, M., Launholt, D., Thomsen, M.S., Nielsen, B.V., Nielsen, H.K. and Merkle, T. (2004) HMGB6 from Arabidopsis thaliana specifies a novel type of plant chromosomal HMGB protein. Biochemistry 43, 1309-1314.

 

2003

Grasser, K.D. (2003) Chromatin-associated HMGA and HMGB proteins: versatile co-regulators of DNA-dependent processes. Plant Mol. Biol. 53, 281-295

Krohn, N.M., Stemmer, C., Fojan, P., Grimm, R. and Grasser, K.D. (2003) Protein kinase CK2 phosphorylates the HMG domain protein SSRP1 inducing the recognition of UV-damaged DNA. J. Biol. Chem. 278, 12710-12715.

Stemmer, C., Leeming, D.J., Franßen, L., Grimm, R. and Grasser, K.D. (2003) Phosphorylation of maize and Arabidopsis HMGB proteins by protein kinase CK2alpha. Biochemistry 42, 3503-3508.

Lichota, J. and Grasser, K.D. (2003) Interaction of maize chromatin-associated HMG proteins with mononucleosomes: role of core and linker histones. Biol. Chem. 384, 1019-1027.

Cavalar, M., Möller, C., Offermann, S., Krohn, N.M., Grasser, K.D. and Peterhänsel, C. (2003) The interaction of DOF transcription factors with nucleosomes depends on the positioning of the binding site and is facilitated by maize HMGB5. Biochemistry 42, 2149-2157.

 

2002

Krohn, N.M., Yanagisawa, S. and Grasser, K.D. (2002) Specificity of the stimulatory interaction between HMGB proteins and the transcription factor Dof2 and its negative regulation by protein kinase CK2-mediated phosphorylation. J. Biol. Chem. 277, 32438-32444.

Stemmer, C., Fernandez, S., Lopez, G., Alonso, J.C. and Grasser, K.D. (2002) Plant chromosomal HMGB proteins efficiently promote the bacterial site-specific ß-mediated recombination in vitro and in vivo. Biochemistry 41, 7763-7770.

Stemmer, C., Schwander, A., Bauw, G., Fojan, P. and Grasser, K.D. (2002) Protein kinase CK2 differentially phosphorylates maize chromosomal HMGB proteins modulating their stability and DNA-interactions. J. Biol. Chem. 277, 1092-1098.

Przewloka, M.R., Wierzbicki, A., Slusarczyk, J., Kuras, M., Grasser, K.D., Stemmer, C. and Jerzmanowski, A. (2002) The male-sterility phenotype induced by changing the proportion of linker histone variants in tobacco is not dependent on the up-regulation of ´drought-inducible´H1s. Planta 215, 371-379.

 

2001

Lichota, J. and Grasser, K.D. (2001) Differential chromatin association and nucleosome binding of the maize HMGA, HMGB and SSRP1 proteins. Biochemistry 40, 7860-786.

 

2000

Jerzmanowski, A., Przewloka, M. and Grasser, K.D. (2000) Linker histones and HMG1 proteins of higher plants. Plant Biol. 2, 586-598.

Röttgers, K., Krohn, N.M., Lichota, J., Stemmer, C., Merkle, T. and Grasser, K.D. (2000) DNA-interactions and nuclear localisation of the chromosomal HMG domain protein SSRP1 from maize. Plant J. 23, 395-405.

Grasser, K.D., Krohn, N.M., Lichota, J., Stemmer, C. (2000) Chromatin-associated HMG1, HMGI/Y and SSRP1 proteins of higher plants. Physiol. Plant. 110, 427-435.

 

1999

Krech, A.B., Wurz, A., Stemmer, C., Feix, G. and Grasser, K.D. (1999) Structure of genes encoding chromosomal HMG1 proteins from maize. Gene 234, 45-50.

Wisniewski, J.R., Krohn, N.M., Heyduk, E., Grasser, K.D. and Heyduk, T. (1999) HMG1 proteins from evolutionary distant organisms distort B-DNA conformation in similar way. Biochim. Biophys. Acta 1447, 25-34.

Stemmer, C., Grimm, R. and Grasser, K.D. (1999) Occurrence of five different chromosomal HMG1 proteins in various maize tissues. Plant Mol. Biol. 41, 351-361.

Krech, A.B., Wulff, D., Grasser, K.D. and Feix, G. (1999) Plant chromosomal HMGI/Y proteins and histone H1 exhibit a protein domain of common origin. Gene 230, 1-5.

 

1998

Grasser K.D. (1998) HMG1 and HU proteins: Architectural elements in plant chromatin. Trends Plant Sci. 3, 260-265.

Ritt, C., Grimm, R. Fernández, S., Alonso, J.C. and Grasser, K.D. (1998) Basic and acidic regions flanking the HMG domain of maize HMGa modulate the interactions with DNA and the self-association of the protein. Biochemistry 37, 2673-2681.

Grimm, R. and Grasser, K.D. (1998) Nanogram scale separations of proteins using capillary HPLC with      fully-automated online microfraction collection followed by protein sequencing, MALDI-TOF/MS and Western blot analysis. J. Chromatography 800, 83-88.

Grasser, K.D1., Teo, S.-H1., Lee, K.-B., Rees, C., Broadhurst, W.R., Hardman, C.H. and Thomas, J.O. (1998) DNA-binding properties of the tandem HMG boxes of high-mobility-group protein 1. Eur. J. Biochem. 253, 787-795.

Ritt, C., Grimm, R., Fernández, S., Alonso, J.C. and Grasser, K.D. (1998) Four differently chromatin-associated maize HMG domain proteins modulate DNA structure and act as architectural elements in nucleoprotein complexes. Plant J. 14, 623-631.

Grimm, R., Grasser, K.D., Kubach, J. and Hancock, B. (1998) A rapid and sensitive procedure for the micro-purification and subsequent characterisation of peptides and protein samples by N-terminal sequencing and matrix assisted laser desorption ionisation time of flight mass spectrometry. Biopharm.Biomed. Anal. 18, 545-554.

Guillén, P., Debón, R.M., Grasser, K.D., Harrsch, P., Grimm, R., Ponte, I. and Palau, J. (1998) Isolation and characterization of a 28 kDa HMG-like protein that binds to A/T-rich distal promoter regions of zein genes. Plant Sci. 135, 31-38.

 

1997

Grasser, K.D., Ritt, C., Krieg, M., Fernández, S., Alonso, J.C. and Grimm, R. (1997) The recombinant product of the Cryptomonas ϕ plastid gene hlpA is an architectural HU-like protein that promotes the assembly of complex nucleoprotein structures. Eur. J. Biochem. 249, 70-76.

Stemmer, C., Ritt, C., Grimm, R., Igloi, G.L. and Grasser, K.D. (1997) Variability in Arabidopsis thaliana chromosomal high-mobility-group-1-like proteins. Eur. J. Biochem. 250, 646-652.

Grasser, K.D., Grimm, R. and Igloi, G.L. (1997) Purification and cDNA cloning of maize HMGd reveal a novel plant chromosomal HMG-box protein with sequence similarity to HMGa. Gene 190, 303-307.

 

1996

Grasser, K.D., Grimm, R. and Ritt, C. (1996) Maize chromosomal HMGc: two closely related structure-specific DNA-binding proteins specify a second type of plant HMG-box protein. J. Biol. Chem. 271, 32900-32906.

 

1995

Teo, S.-H., Grasser, K.D., Hardman, C.H., Broadhurst, R.W., Laue, E.D. and Thomas, J.O. (1995) Two mutations in the HMG-box  with very different structural consequences provide insights into the nature of binding to four-way junction DNA. EMBO J. 14, 3844-3853.

Hardman, C.H., Broadhurst, R.W., Raine, A.R.C., Grasser, K.D., Thomas, J.O. and Laue, E.D. (1995) Structure of the A-domain of HMG1 and its interaction with DNA as studied by heteronuclear three- and four-dimensional NMR-spectroscopy. Biochemistry 34, 16596-16607.

Teo, S.-H., Grasser, K.D. and Thomas, J.O. (1995) Differences in the DNA-binding properties of the HMG-box domains in HMG1 and SRY. Eur. J. Biochem. 230, 943-950.

Grasser, K.D. (1995) Plant chromosomal high mobility group (HMG) proteins. Plant J. 7, 185-192.

 

1994

Grasser, K.D., Krech, A.B. and Feix, G. (1994) The maize chromosomal HMGa protein recognizes structural features of DNA and increases DNA flexibility. Plant J. 6, 351-358.

Grasser, K.D., Hetz, W. and Feix, G. (1994) Stability of the maize chromosomal high-mobility-group proteins, HMGa and HMGb, in vivo. Plant Mol. Biol. 25, 565-568.

 

1993

Grasser, K.D., Hetz, W., Griess, E.A. and Feix, G. (1993) Stimulatory effect of the maize HMGa protein on reporter gene expression in maize protoplasts. FEBS Lett. 327, 141-144.

Griess, E.A., Grasser, K.D. and Feix, G. (1993) Repeat units from a maize rDNA external spacer region exhibit DNA-curvature and interact with HMG proteins. Planta 191, 524-531.

Grasser, K.D., Wohlfarth, T., Bäumlein, H. and Feix, G. (1993) Comparative analysis of chromosomal HMG proteins from monocotyledons and dicotyledons. Plant Mol. Biol. 23, 619-625.

Griess, E.A., Rensing, S.A., Grasser, K.D., Maier, U.-G. and Feix, G. (1993) Phylogenetic relationships of the HMG box DNA-binding domains. J. Mol. Evol. 37, 204-210.

 

1992

Grasser, K.D., Haass, M.M., Griess, E.A. and Feix, G. (1992) Detection of the chromosomal HMGa protein in crude maize extracts. J. Plant Physiol. 140, 634-636.

 

1991

Grasser, K.D. and Feix, G. (1991) Isolation and characterization of maize cDNAs encoding a high mobility group protein displaying a HMG-box. Nucleic Acids Res. 19, 2573-2577.

Grasser, K.D., Wurz, A. and Feix, G. (1991) Isolation and characterization of high mobility group (HMG) proteins from maize. Planta 185, 350-355.

 

1990

Grasser, K.D., Maier, U.-G., Haass, M.M. and Feix, G. (1990) Maize high mobility group proteins bind to CCAAT and TATA Boxes of a zein gene promoter. J. Biol. Chem. 265, 4185-4188.

Maier, U.-G., Grasser, K.D., Haass, M.M. and Feix, G. (1990) Multiple proteins bind to the promoter region of the zein gene pMS1 of maize. Mol. Gen. Genet. 221, 164-170.

 

1989

Grasser, K.D., Maier, U.-G. and Feix, G. (1989) A nuclear casein type II kinase from maize endosperm phosphorylating HMG proteins. Biochem. Biophys. Res. Commun. 162, 456-463.




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  1. Fakultät für Biologie und Vorklinische Medizin
  2. Faculty Research

Grasser lab

Cell Biology & Plant Biochemistry
University of Regensburg

 

 

Rechter Rand 6


Universitätsstrasse 31
93053 Regensburg, Germany
 

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