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Kompetenzzentrum Fluoreszente Bioanalytik

 

Kfb Staff

 

The Kompetenzzentrum Fluoreszente Bioanalytik (KFB) is a genomics core facility based at the University of Regensburg. The facility supports state-of-the-art genomics services for the local research community, as well as for external partners and clients across Europe.

Since 2001, our facility has contributed to more than 200 publications in peer-reviewed journals. Click here for an up-to-date list.

 

Our lab is equipped with the following platform technologies:

  • GeneChip microarrays (Affymetrix – now part of Thermo Fisher): RNA expression profiling using single-cartridge arrays (GeneChip Scanner 3000), or PEG-plate arrays (GeneTitan system).
  • Deep Sequencing (Illumina NextSeq 500; Illumina HiSeq 1000): a growing range of sequencing applications and protocols are supported, including mRNA-Seq, total RNA-Seq, gDNA-Seq, ChIP-Seq, smallRNA-Seq.

 
In addition, we offer stand-alone access to the following instruments:          
StepOne Plus Real-Time PCR System (Thermo Fisher)

  • Agilent Bioanalyzer 2100 (DNA / RNA QC)
  • Covaris S2 AFA (highly-reproducible DNA fragmentation; recommended in many different sequencing library prep protocols)
  • Precellys 24 Homogenisator (bead-based disruption/homogenization of a wide range of tissues)
  • Nanodrop 1000 Spectrophotometer (DNA / RNA quantification)

Our experienced staff provides support along the full experimental workflow: e. g. project design/counseling, cost estimation/preparation of quotes, choice of kits and protocols, sample QC, sample preparation, data QC, and data analysis.

KFB is a member of the SFB960  and a cooperation partner of the Regensburger Centrum für Interventionelle Immunologie (RCI).

 

Sample submission:

Please bring in samples, or send them on dry ice. Don’t forget to fill in and sign the corresponding order form:

HiSeq sequencing service (english / german)

NextSeq sequencing service (english / german)

GeneChip (Affymetrix) microarray service (english / german)

 

Pricing:
Please contact us (Email: info@kfb-regensburg.de) for pricing, as charges vary per institutional affiliation, and depending on the payment model (fee-for-service, or cooperative).

 

Acknowledgements:

Researchers who use our Affymetrix microarray service on a fee-for-service basis must acknowledge KFB as follows: “Sample processing and Affymetrix microarray hybridization were carried out at a genomics core facility: Center of Excellence for Fluorescent Bioanalytics (KFB, University of Regensburg, Germany).”

Researchers who use our Illumina Deep Sequencing services on a fee-for-service basis must acknowledge KFB as follows: “Illumina deep sequencing was carried out at a genomics core facility: Center of Excellence for Fluorescent Bioanalytics (KFB, University of Regensburg, Germany).”

 

Terms of use:
Please download our Nutzungsordnung (.pdf).

 

  1. STARTSEITE UR

Kompetenzzentrum Fluoreszente Bioanalytik

Dr. Thomas Stempfl

 

Thomas Stempfl

 

Tel. 0941/943-5011
Fax: 0941/943-5018
info@kfb-regensburg.de