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Microbiology

Focus Subject - Microbiology

You have to choose the theoretical module and one practical module.

TM  Theoretical Module (12 CP)

Microbiology

Prof. Dr. Dina Grohmann

PM  Practical Modules (12 CP each)

Organismic Microbiology Prof. Dr. Reinhard Rachel
Microbial Cell Biology Prof. Dr. Reinhard Rachel
Chemical Biology and Single-Molecule Biochemistry Prof. Dr. Dina Grohmann
Molecular Microbiology PD Dr. Winfried Hausner

TM Microbiology

BIO-M-TM-M

Detailed module description - pdf

Prof. Dr. Dina Grohmann

Module contents
The lectures cover the following topics:
  • Up-to-date taxonomy of the Prokaryotes;
  • Methods of modern systematics (especially –omis-based models of relationships);
  • problems and limits of cultivation and taxonomy;
  • Presentation of the important archaeal and bacterial groups
  • Structural Biology of microbial cells (Bacteria, Archaea)
  • Gene regulation in Prokaryotes with specific transcription factors or alternative sigma factors
  • Methods to explore gene regulation systems
  • Posttranscriptional regulation with small RNAs and special RNA-binding proteins
  • Organisation and mechanisms of the CRISPR-Cas sytems
  • Modern fluorescence-based methods (e.g. single-molecule FRET, superresolution microscopy) to study biomolecules in vivo and in vitro

In the seminar, current research topics will be presented and discussed.
Topics include:
  • microbial diversity, methods in microbial ecology and microbial-driven processes of environmental and/or biotechnological relevance
  • molecular microbiology with a special focus on transcriptional and posttranscriptional gene regulation
Module components
  1. at least two lectures in the field of microbiology (for a selection see LSF); e.g. Systematic of the Prokaryotes, Gene regulation in Prokaryotes.
  2. one seminar in the field of microbiology (for a selection see LSF); e.g. Environmental Microbiology and Biotechnology or Molecular Microbiology

In the seminar a seminar presentation has to be held and registered in Flexnow as an ungraded study achievement (Studienleistung).

Module examination

30 min oral examination (graded)


PM Organismic Microbiology

BIO-M-PM-OM

Detailed module description - pdf

Prof. Dr. Reinhard Rachel (link to research group)

Module contents
  • Intensive knowledge transfer in the field of organismic microbiology
  • Enrichment and isolation of Bacteria and Archaea from a variety of original
      samples like sediments, soils, hot springs, black smokers, or animal digestive tracts
  • physiological, biochemical and structural characterization of the isolates
  • learning of a broad spectrum of cultivation techniques (e.g. anaerobic technique;
      working with hyperthermophiles)
  • learning of special isolation techniques (e.g. optical tweezers),
  • introduction to the ultrastructural characterization of isolates by electron microscopy
  • molecular characterization of the isolates (e.g. sequencing of the 16S rRNA genes).
  • Presentation and discussion of own experimental gained data
Module components

Laboratory course (6 weeks) with seminar: Organismic Microbiology

Module examination

Portfolio examination (graded)


PM Microbial Cell Biology

BIO-M-PM-MCB

Detailed module description - pdf

Prof. Dr. Reinhard Rachel (link to research group)

Module contents
  • Intensive knowledge transfer in the field of structural characterization of microbes
  • visualization of intact cells (Bacteria; Archaea) using different preparation methods for electron microscopy
  • ultrastructural characterization of the isolates: heavy-metal shadowing, freeze-etching, ultrathin sections
  • learning of a broad spectrum of EM techniques (e.g. negative staining;
    freeze-etching, cryoTEM)
  • learning of special data recording techniques (e.g. electron tomography; cryoTEM),
  • introduction to image processing for electron tomography
  • Presentation and discussion of own experimental gained data
Module components

Laboratory course (6 weeks) with seminar: Microbial Cell Biology

Module examination

Portfolio examination (graded)


PM Chemical Biology and Single-Molecule Biochemistry

BIO-M-PM-CBSMB

Detailed module description - pdf

Prof. Dr. Dina Grohmann (link to research group)

Module contents
  • Advanced insights in the field of fluorescence-based analysis of biomolecules
  • Theoretical and practical knowledge of single-molecule methods in biological research with special emphasis on fluorescence-based techniques like single-molecule FRET, single-molecule pulldown, fluorescence correlation spectroscopy (FCS) using confocal and TIRF-based microscopes
  • Approaches for the site-specific incorporation of fluorescent dyes into proteins
  • Incorporation of unnatural amino into prokaryotic and eukaryotic proteins for site-specific modification
  • Presentation and discussion of own experimental gained data
Module components

Laboratory course (6 weeks) with seminar: Chemical Biology and Single-Molecule Biochemistry

Module examination

Portfolio examination (graded)


PM Molecular Microbiology

BIO-M-PM-MoM

Detailed module description - pdf

PD Dr. Winfried Hausner (link to research group)

Module contents
  • Intensive knowledge transfer in the field of molecular microbiology
  • Theoretical and practical knowledge about different molecular standard techniques (Isolation of DNA, PCR, cloning, Southern blot Western blot)
  • Heterologous expression of proteins
  • Chromatographic purification of proteins
  • Methods for the analysis of enzymatic activities (e.g. polymerases, nucleases, RNA-Chaperons)
  • Methods for analysis of DNA-protein and RNA-protein interactions using the archaeal transcription machinery and prokaryotic CRISPR-Cas systems as example
  • Presentation and discussion of own experimental gained data
Module components

Laboratory course (6 weeks) with seminar: Molecular Microbiology

Module examination

Portfolio examination (graded)

  1. Fakultät für Biologie und Vorklinische Medizin
  2. Studium