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Lehrstuhl Mikrobiologie, Grohmann-Publications

Fakultät für Biologie und Vorklinische Medizin
Institut für Biochemie, Genetik und Mikrobiologie
Lehrstuhl für Mikrobiologie & Archaeenzentrum
Prof. Dr. Dina Grohmann - Publications


Willkomm S., Makaraova K. and Grohmann D. (2018). Prokaryotic Argonaute proteins represent an additional layer in prokaryotic defence. FEMS Reviews Microbiology (epub ahead of print), https://doi.org/10.1093/femsre/fuy010

Gust A., Leonhard J., Zeitler D., Willkomm S., Kramm K., Tinnefeld P., Meister G. and Grohmann D. (2018). Site-specific fluorescent labelling of endogenous mammalian proteins for single-molecule FRET analysis. ChemBioChem (epub ahead of print), https://doi.org/10.1002/cbic.201700696


Jerome Gouge, Nicolas Guthertz, Kevin Kramm, Oleksandr Dergai, Guillermo Abascal-Palacios, Karishma Satia, Pascal Cousin, Nouria Hernandez, Dina Grohmann & Alessandro Vannini (2017).Molecular mechanisms of Bdp1 in TFIIIB assembly and RNA polymerase III transcription initiation. Nature communications

Zander A., Willkomm S., Ofer S., van Wolferen M., Egert L., Buchmeier S., Stöckl S., Tinnefeld P., Schneider S., Klingl A., Albers S.-V., Werner F. and Grohmann D.* (2017). Guide-independent DNA cleavage by archaeal Argonaute from Methanocaldococcus jannaschii. Nature Microbiology (epub ahead of print)

Willkomm S., Oellig C. A., Zander A., Restle T., Keegan R., Grohmann D.* and Schneider, S.* (2017). Structural and mechanistic insights into an archaeal DNA-guided Argonaute protein. Nature Microbiology (epub ahead of print)

Blombach F. and Grohmann D. (2017). Same same but different: the evolution of TBP in archaea and their eukaryotic offspring. Transcription. (accepted)


Willkomm S., Zander A. and Grohmann D. (2017) Site-Specific Fluorescent Labeling of Argonaute for FRET-based Bio-Assays. Methods in Molecular Biology, 1517:291-304

Kucher S, Korneev S. Tyagi S., Apfelbaum R., Grohmann D., Lemke E.A., Klare, J., Steinhoff H.-J., Klose, D. (2016) Orthogonal spin labelling using click chemistry for in vitro and in vivo applications. Journal of Magnetic Resonance, 275:38-45

Sheppard C., Blombach F., Belsom A., Schulz S., Daviter T., Smollett K., Mahieu E., Erdmann S., Tinnefeld P., Garrett R., Grohmann D., Rappsilber J., Werner F. (2016) Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP. Nature Communications, 7:13595

Nickels P.C., Wünsch B., Holzmeister P., Bae W., Kneer L.M., Grohmann D., Tinnefeld P. and Liedl T. (2016), Molecular Force Spectroscopy with a DNA Origami-Based Nanoscopic Force Clamp. Science. 354(6310):305-307.

Willkomm S., Zander A., Grohmann D and Restle T. (2016). Mechanistic insights into archaeal and human Argonaute substrate binding and cleavage properties. PLOS one, 11(10):e0164695

Blombach B., Smollett K.L., Grohmann D. and Werner F. (2016). Molecular Mechanisms of Transcription Initiation – structure function and evolution of TFE/TFIIE-like factors and open complex formation. Journal of Molecular Biology (epub, ahead of print)

Schulz S., Gietl A., Smollett K., Tinnefeld P., Werner F. and Grohmann D. (2016). TFE and Spt4/5 open and close the RNA polymerase clamp during the transcription cycle. Proceedings of the National Academy of Sciences U.S.A. (epub, ahead of print)

Jakob L., Treiber T., Treiber N., Gust A., Kramm K., Hansen K., Stotz M., Wankerl L., Herzog F., Hannus S., Grohmann D., and Meister G. (2016). Structural and functional insights into the fly microRNA biogenesis factor Loquacious. RNA doi: 10.1261/rna.055426.115. (published in advance January 14, 2016).

Molle J., Raab M., Holzmeister S., Schmitt-Monreal D., Grohmann D., He Z., Tinnefeld P. (2016). Superresolution microscopy with transient binding. Current Opinion in Biotechnology, 39: 8-16.


Schulz S., Kramm K., Werner F., Grohmann D. (2015). A fluorescently labeled recombinant RNAP system to probe archaeal transcription initiation. Methods (epub, ehead of print) (cover figure)

Willkomm S., Zander A., Gust A., Grohmann D. (2015). A prokaryotic twist on Argonaute action. Life. 5 (1), 538-553.

Nagy J., Grohmann D., Cheung A.C.M., Schulz S., Smollett K., Werner F. & Michaelis J. (2015): Complete architecture of the archaeal RNA polymerase open complex from single-molecule FRET and NPS, Nature Communications, 6:6161


A. Gust, A. Zander, A. Gietl, P. Holzmeister, S. Schulz, B. Lalkens, P. Tinnefeld, D. Grohmann (2014): A Starting Point for Fluorescence-Based Single-Molecule Measurements in Biomolecular Research - Molecules, 19, 10, 15824-15865.

Acuna G., Grohmann D., Tinnefeld P. (2014) Enhancing single-molecule fluorescence with nanophotonics. FEBS letters

Gietl A., Holzmeister P., Blombach F., Schulz S., Voithenberg L.V.v., Lamb D., Werner F., Tinnefeld P., Grohmann D. (2014) Single-molecule analysis reveals diverse pathways during early steps in transcription initiation. Nucleic Acids Research. 42(10):6219-31. Selected by the Faculty 1000

Zander A., Holzmeister P., Klose D., Tinnefeld P. and Grohmann D. (2014). Single-molecule FRET supports the two-state model of Argonaute action. RNA Biology 11(1):45-56. (featured image from our article)

Afanasyeva A., Hirtreiter A., Schreiber A., Grohmann D., Pobegalov E., McKay A.R., Tsaneva I., Petukhov M., Käs E., Grigoriev M. and Werner F. (2014). Lytic water dynamics reveal evolutionarily conserved mechanisms of ATP hydrolysis by TIP49 AAA+ ATPases. Structure. 22(4):549-59.

Holzmeister P., Acuna G.P., Grohmann D. and Tinnefeld P. (2014) Breaking the Concentration Limit of Optical Single-Molecule Detection. Chemical Society Reviews 43(4):1014-28. (back cover)


P. Holzmeister, G.P. Acuna, D. Grohmann, P. Tinnefeld (2013): Breaking the Concentration Limit of Optical Single -Molecule Detection - Chem.Soc.Rev., online.

Grohmann D., Werner F. and Tinnefeld P. (2013). Making Connections -- Strategies for Single Molecule Fluorescence Biophysics. Current Opinion in Chemical Biology. 17(4):691-8.

Gietl A. and Grohmann D. (2012). Modern biophysical approaches probe transcription factor induced DNA bending and looping. Biochem Soc Trans. 41(1):368-73.

Blombach F, Daviter T, Fielden D, Grohmann D, Smollett K, Werner F. (2013): Archaeology of RNA polymerase: factor swapping during the transcription cycle. - Biochem Soc Trans. 1;41(1):362-7


Blombach F., Daviter T., Fielden D., Grohmann D., Smollett K. and Werner F. (2012). Archaeology of RNA polymerase -- factor swapping during the transcription cycle. Biochem Soc Trans. 41(1):362-7.

Klose D., Klare J., Grohmann D., Kay C.W.M., Werner F. and Steinhoff H-J. (2012). Simulation vs. reality: a comparison of in silico predictions with DEER and FRET measurements. PLOS one, 7(6):e39492.

Gietl A., Holzmeister P., Grohmann D. and Tinnefeld P. (2012). DNA origami as biocompatible surface to match single-molecule and ensemble experiments. Nucleic Acids Research, epub.


Grohmann D., Nagy J., Chakraborty A., Klose D., Fielden D., Ebright R.H., Michaelis J., Werner F. (2011). The Initiation Factor TFE and the Elongation Factor Spt4/5 Compete for the RNAP Clamp during Transcription Initiation and Elongation. Molecular Cell, 43:263-74.

Grohmann D. and Werner F. (2011) Recent advances in the understanding of archaeal transcription. Curr Opin Microbiol.

Ryan C.P., Smith M.E., Schumacher F.F., Grohmann D., Papaioannou D., Waksman G., Werner F., Baker J.R., Caddick S. (2011) Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis. Chem Commun. 47:5452-4.

Grohmann D., Klose D., Fielden D., Werner F. (2011) FRET (fluorescence resonance energy transfer) sheds light on transcription. Biochem Soc Trans. 39:122-7.

Werner F. and Grohmann D. (2011) Evolution of multisubunit RNA polymerases in the three domains of life. Nature Reviews Microbiol.9:85-98.

Grohmann D. and Werner F. (2011) Cycling through Transcription with the RNA polymerase F/E (RPB4/7) Complex - Structure, Function and Evolution of Archaeal RNA polymerase., Research in Microbiology 162:10-8.


Grohmann D., Klose D., Klare J.P., Kay C.W., Steinhoff H.J., Werner F. (2010) RNA-binding to archaeal RNA polymerase subunits F/E: a DEER and FRET study. J Am Chem Soc., 132:5954-5.

Hirtreiter A., Damsma G.E., Cheung A.C., Klose D., Grohmann D., Vojnic E., Martin A.C., Cramer P., Werner F. (2010), Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif. Nucleic Acids Research 38:4040-51.

Hirtreiter A., Grohmann D., Werner F. (2010) Molecular mechanisms of RNA polymerase -- the F/E (Rpb4/7) complex is required for high processivity in vitro. Nucleic Acids Research, 38:585-96.

Grohmann, D. & Werner, F. (2010) Hold on!: RNA polymerase interactions with the nascent RNA modulate transcription elongation and termination. RNA Biology,  7: 310-315.


Grohmann D., Hirtreiter A., Werner F. (2009), RNAP subunits F/E (Rpb4/7) are stably associated with archaeal RNA polymerase: using fluorescence anisotropy to monitor RNAP assembly in vitro. Biochem J, 421:339-43. (selected by the Faculty 1000)

Grohmann D., Hirtreiter A., Werner F. (2009), Molecular mechanisms of archaeal RNA polymerase. Biochem Soc Trans. 37:12-7.


Grohmann D., Godet J., Mely Y., Darlix JL. and Restle, T. (2008).  HIV-1 NC traps the reverse transcriptase on primer/template substrates. Biochemistry, 47:12230-40.

Di Pasquale F, Fischer D, Grohmann D, Restle T, Geyer A, Marx A. (2008) Opposed steric constraints in human DNA polymerase beta and E.coli DNA polymerase I. J Am Chem Soc 130:10748-57.

Grohmann D., Corradi V., Horenkamp F., Laufer, S.D., Manetti, F. Botta, M. and Restle, T. (2008).  Small molecule inhibitors targeting HIV-1 dimerization. Chembiochem 9:916-22.


Yamazaki S., Tan L., Mayer G., Hartig J.S., Song J.N., Reuter S., Restle T., Laufer S.D., Grohmann D., Kräusslich H.G., Bajorath J., Famulok M. (2007). Aptamer displacement identifies alternative small-molecule target sites that escape viral resistance. Chem Biol.14:804-12.


Mescalchin A., Wünsche W., Laufer S.D., Grohmann, D., Restle, T. and Sczakiel, G. (2006).  Specific binding of a bioactive hexanucleotide to HIV-1 reverse transcriptase: a novel class of small oligomeric nucleic acid drugs. Nucleic Acids Research, 34, 5631--5637.


Plücken H, Müller B., Grohmann D., Westhoff P., Eichacker LA. (2002). The HCF136 protein is essential for assembly of the photosystem II reaction center in Arabidopsis thaliana. FEBS Letters 532:85-90.

  1. Fakultät für Biologie und Vorklinische Medizin
  2. Faculty Research

Lehrstuhl für Mikrobiologie & Archaeenzentrum

Prof. Dr. Dina Grohmann


Lehrstuhl für Mikrobiologie
Universitätsstraße 31
93053 Regensburg

Phone: +49 941 9433147
Fax: +49 941 9432403
Mail: dina.grohmann@ur.de