Oxidases convert their substrates under the release of HP. The HP level is directly related to the amount of substrate that is present in the sample or the amount of product that has been formed. Hence, concentrations of substrates or products of oxidases can be quantified by probes for HP. This opens access to a variety of bioanalytes like glucose, lactate, uric acid, sarcosine, superoxide radicals, xanthine, alcohols and amines. We have therefore created new fluorescence assays using the HP Green dye that were the most sensitive ones (at the time of their publication) with incubation times of only few minutes
Selected publications:
Groegel DBM, Link M, Duerkop A*, Wolfbeis OS, 2011, A New Fluorescent PET Probe for Hydrogen Peroxide and its Use in Enzymatic Assays for L-Lactate and D-Glucose, ChemBioChem, 12:2779-2785, DOI: 10.1002/cbic.201100561 (externer Link, öffnet neues Fenster)
Wu M, Lin Z, Schäferling M, Dürkop A, Wolfbeis OS, 2005, Fluorescence Imaging of the Activity of Glucose Oxidase Using a Hydrogen Peroxide Sensitive Europium Probe, Anal Biochem, 340:66-73, DOI: 10.1016/j.ab.2005.01.050 (externer Link, öffnet neues Fenster)
Wolfbeis O. S., Schäferling M., Dürkop A., 2003, Reversible Optical Sensor Membrane for Hydrogen Peroxide Using an Immobilized Fluorescent Probe, and its Application to a Glucose Biosensor, Microchim Acta, 143:221-227, DOI: 10.1007/s00604-003-0090-5 (externer Link, öffnet neues Fenster)