We create dipsticks by embedding suitable probes into polymer matrices coated onto a solid support. Common application is the quantitation of biogenic amines as indicators of food freshness. Concentrations of BAs that induce food poisoning but are not perceived by the human nose are displayed by red color. In order to keep the readout simple, photographic images of the sticks are taken by a digital camera using luminescence or reflectometric evaluation.
More recent developments comprised dipsticks for ion analysis or for detection of chemical weapons like sulfur mustard. All readouts can be performed either by digital photography with low instrumental demand or in microtiter plates. Analytes relevant for the development of new dipsticks are alcohols, aldehydes, ketones, pH and heavy metal ions.
Selected publications:
Danchuk A, Komova NS, Mobarez SN, Doronin SY, Burmistrova NA, Markin AV Duerkop A, 2020, Optical Sensors for Determination of Biogenic Amines in Food, Anal Bioanal Chem, 412:4023-4036, DOI: 10.1007/s00216-020-02675-9 (externer Link, öffnet neues Fenster)
Khairy GM, Duerkop A, 2019, Dipsticks and Sensor Microtiterplate for Determination of Copper(II) in Drinking Water Using Reflectometric RGB Readout of Digital Images, Fluorescence or Eye-Vision, Sens Actuat B, 281:878–884, DOI: 10.1016/j.snb.2018.10.147 (externer Link, öffnet neues Fenster)
Bidmanova S, Steiner M-S, Stepan M, Vymazalova K, Gruber MA, Duerkop A, Damborsky J, Prokop Z, Wolfbeis OS, 2016, Enzyme based test stripes for the visual or reflectometric detection and quantitation of gaseous sulfur mustard, Anal Chem 88:6044-6049, DOI: 10.1021/acs.analchem.6b01272 (externer Link, öffnet neues Fenster)
Steiner M-S, Meier, RJ, Duerkop A, Wolfbeis OS, 2010, Chromogenic Sensing of Biogenic Amines Using a Chameleon Probe and the Red Green Blue Readout of Digital Camera Images, Anal Chem, 82:8402-8405, DOI: 10.1021/ac102029j (externer Link, öffnet neues Fenster)