Publications

Helmbrecht V, Reichelt R, Grohmann D, Orsi WD. Simulated early Earth geochemistry fuels a hydrogen-dependent primordial metabolism.
Nat Ecol Evol. 2025 May;9(5):769-778. doi: (external link, opens in a new window) 10. 1038/s41559-025-02676-w (external link, opens in a new window). Epub 2025 Apr 30.

Grohmann D, Grünberger F. Single-molecule sequencing of RNA.
American Chemical Society. 2025 April 24; doi: 10.1021/acsinfocus.7e9002 (external link, opens in a new window)

Hanelt TN, Treiber N, Treiber T, Lehmann G, Eichner N, Rothmeier T, Schmid G, Reichelt R, Zambelli F, Pavesi G, Grohmann D, Meister G. Endo-bind-n-seq: identifying RNA motifs of RNA binding proteins isolated from endogenous sources.
Life Sci Alliance. 2024 Dec 2;8(2):e202402782. doi: 10.26508/lsa.202402782 (external link, opens in a new window)

Tarau D, Grünberger F, Pilsl M, Reichelt R, Heiß F, König S, Urlaub H, Hausner W, Engel C, Grohmann D. Structural basis of archaeal RNA polymerase transcription elongation and Spt4/5 recruitment.
Nucleic Acids Research, gkae282, https://doi.org/10.1093/nar/gkae282 (external link, opens in a new window)

Vornholt T, Joncev Z, Sabatino V, Panke S, Ward T.R, Sparr C, Jeschek M. An artificial metalloenzyme for atroposelective metathesis. ChemCatChem e202301113. doi:10.1002/cctc.202301113 (external link, opens in a new window)

Grünberger F, Schmid G, El Ahmad Z, Fenk M, Vogl K, Reichelt R, Hausner W,
Urlaub H, Lenz C, Grohmann D. Uncovering the temporal dynamics and regulatory
networks of thermal stress response in a hyperthermophile using transcriptomics
and proteomics. mBio. 2023 Oct 16:e0217423. doi: 10.1128/mbio.02174-23 (external link, opens in a new window)

Kramm K, Schröder T, Vera AM, Grabenhorst L, Tinnefeld P, Grohmann D. DNA Origami-Based Single-Molecule Force Spectroscopy and Applications. Methods Mol Biol. 2024;2694:479-507. doi: 10.1007/978-1-0716-3377-9_23 (external link, opens in a new window)

Reichelt R, Rothmeier T, Grünberger F, Willkomm S, Bruckmann A, Hausner W, Grohmann D. The archaeal Lsm protein from Pyrococcus furiosus binds co-transcriptionally to poly(U)-rich target RNAs. Biol Chem. 2023 Sep 15.
doi: 10.1515/hsz-2023-0215 (external link, opens in a new window)

Baes R, Grünberger F, Pyr Dit Ruys S, Couturier M, De Keulenaer S, Skevin S, Van Nieuwerburgh F, Vertommen D, Grohmann D, Ferreira-Cerca S, Peeters E. Transcriptional and translational dynamics underlying heat shock response in the thermophilic crenarchaeon Sulfolobus acidocaldarius. mBio. 2023 Aug 29:e0359322.
doi: 10.1128/mbio.03593-22 (external link, opens in a new window)

Agam G, Gebhardt C, Popara M, Mächtel R, Folz J, Ambrose B, Chamachi N, Chung SY, Craggs TD, de Boer M, Grohmann D, Ha T, Hartmann A, Hendrix J, Hirschfeld V, Hübner CG, Hugel T, Kammerer D, Kang HS, Kapanidis AN, Krainer G, Kramm K, Lemke EA, Lerner E, Margeat E, Martens K, Michaelis J, Mitra J, Moya Muñoz GG, Quast RB, Robb NC, Sattler M, Schlierf M, Schneider J, Schröder T, Sefer A, Tan PS, Thurn J, Tinnefeld P, van Noort J, Weiss S, Wendler N, Zijlstra N, Barth A, Seidel CAM, Lamb DC, Cordes T. Reliability and accuracy of single-molecule FRET studies for characterisation of structural dynamics and distances in proteins. Nat Methods. 2023 Apr;20(4):523-535.
doi: 10.1038/s41592-023-01807-0 (external link, opens in a new window)

Seeholzer T, Tarau D, Hollendonner L, Auer A, Rachel R, Grohmann D, Giessibl FJ, Weymouth AJ. A Next-Generation qPlus-Sensor-Based AFM Setup: Resolving Archaeal S-Layer Protein Structures in Air and Liquid. J Phys Chem B. 2023 Aug 10;127(31):6949-6957. doi: 10.1021/acs.jpcb.3c02875 (external link, opens in a new window)

Dengler L, Meier J, Klingl A, Nißl L, Bellack A, Grohmann D, Rachel R, Huber H. A novel interdomain consortium from a Costa Rican oil well composed of Methanobacterium cahuitense sp. nov. and Desulfomicrobium aggregans sp. nov.
Arch Microbiol. 2023 Apr 13;205(5):189. doi: (external link, opens in a new window) 10. 1007/s00203-023-03533-9 (external link, opens in a new window).

Grünberger F, Jüttner M, Knüppel R, Ferreira-Cerca S*, Grohmann D.* Nanopore-based RNA sequencing deciphers the formation, processing, and modification steps of rRNA intermediates in archaea. RNA. 2023 Aug;29(8):1255-1273.
doi:10.1261/rna.079636.123 (external link, opens in a new window)

S. Höllerer, M. Jeschek. Ultradeep characterisation of translational sequence determinants refutes rare-codon hypothesis and unveils quadruplet base pairing of initiator tRNA and transcript.
Nucleic Acids Research gkad040, https://doi.org/10.1093/nar/gkad040 (external link, opens in a new window)

Kaul A., Böllmann A., Thema M., Kalb L., Stöckl R., Huber H., Sterner M., Bellack A. (2022). Combining a robust thermophilic methanogen and packing material with high liquid hold-up to optimise biological methanation in trickle-bed reactors.
Bioresource Technology 345:126524. doi: 10.1016/j.biortech.2021.126524 (external link, opens in a new window)

Dengler L, Meier J, Grünberger F, Bellack A, Rachel R, Grohmann D, Huber H. Methanofollis propanolicus sp. nov., a novel archaeal isolate from a Costa Rican oil well that uses propanol for methane production. Arch Microbiol. 2022 Aug 13;204(9):554.
doi: 10.1007/s00203-022-03152-w (external link, opens in a new window)

Gambelli L., Isupov M. N., Conners R., McLaren M., Bellack A., Gold V., Rachel R., Daum B. (2022). An archaellum filament composed of two alternating subunits.
Nature Communications 13:710. doi: 10.1038/s41467-022-28337-1 (external link, opens in a new window)

Willkomm S., Jakob L., Kramm K., Graus V., Neumeier J., Meister G., Grohmann D. (2022). Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2.
Nat Commun 13, 3825. doi.org/10.1038/s41467-022-31480-4 (external link, opens in a new window)

Buriánková, I.; Molíková, A.; Vítězová, M.; Onderka, V.; Vítěz, T.; Urbanová, I.; Hanišáková, N.; Černý, M.; Novák, D.; Lochman, J.; Zeman, J.; Javůrek, J.; Machálková, M.; Dengler, L.; Huber, H. (2022). Microbial Communities in Underground Gas Reservoirs Offer Promising Biotechnological Potential.
Fermentation 2022, 8, 251. https://doi.org/10.3390/fermentation8060251 (external link, opens in a new window)

S. Höllerer, M. Jeschek. (2022). Ultradeep characterisation of translational sequence determinants refutes rare-codon hypothesis and unveils quadruplet base pairing of initiator tRNA and transcript.
bioRxiv doi: https://doi.org/10.1101/2022.05.02.490318 (external link, opens in a new window)

Wörle E, Newman A, D'Silva J, Burgio G, Grohmann D. Allosteric activation of CRISPR-Cas12a requires the concerted movement of the bridge helix and helix 1 of the RuvC II domain.
Nucleic Acids Res. 2022 Sep 23;50(17):10153-10168. doi:10.1093/nar/gkac767 (external link, opens in a new window)

Grünberger F., Ferreira-Cerca S. and Grohmann D. (2022) Nanopore sequencing of RNA and cDna molecules in Escherichia coli. RNA 28(3): 400-417.
doi: 10.1261/rna.078937.121 (external link, opens in a new window)

2021

Thema M., Weidlich T., Kaul A., Böllmann A., Huber H., Bellack A., Karl J., Sterner M. (2021). Optimised biological CO2-methanation with a pure culture of thermophilic methanogenic archaea in a trickle-bed reactor.
Bioresource Technology 333:125135. doi: 10.1016/j.biortech.2021.125135 (external link, opens in a new window)

T. Vornholt, F. Christoffel, M. Pellizzoni, S. Panke, T.R. Ward, M. Jeschek. (2021). Systematic engineering of artificial metalloenzymes for new-to-nature reactions.
Science Advances 7:eabe4208, https://www.science.org/doi/pdf/10.1126/sciadv.abe4208 (external link, opens in a new window)

Wörle E., Jakob L., Schmidbauer A., Zinner G., Grohmann D. (2021). Decoupling the bridge helix of Cas12a results in a reduced trimming activity, increased mismatch sensitivity and impaired conformational transitions.
Nucleic Acids Research, https://doi.org/10.1093/nar/gkab286 (external link, opens in a new window)

Weixlbaumer A., Grünberger F., Werner F. and Grohmann D. (2021). Coupling of transcription and translation in archaea: cues from the bacterial world.
Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2021.661827 (external link, opens in a new window)

Grünberger F., Reichelt R., Waege I., Niedermaier V., Bronner K., Kaljanac M., Weber N., El Ahmad Z., Madej G., Knauss L., Ziegler C., Grohmann D., Hausner W. (2021) CopR, a global regulator of transcription to maintain copper homeostasis in Pyrococcus furiosus. Frontiers in Microbiology. doi:10.3389 (external link, opens in a new window)

2020

T. Vornholt, M. Jeschek. (2020) The quest for xenobiotic enzymes - from new enzymes for chemistry to a novel chemistry of life.
ChemBioChem, 21:2241-2249. https://www.science.org/doi/10.1126/sciadv.abe4208 (external link, opens in a new window)

S. Höllerer, L. Papaxanthos, A.C. Gumpinger, K. Fischer, C. Beisel, K. Borgwardt, Y. Benenson, M. Jeschek. (2020) Large-scale DNA-based phenotypic recording and deep learning enable highly accurate sequence-function mapping.
Nature Communications 11:3551. https://www.nature.com/articles/s41467-020-17222-4 (external link, opens in a new window)

Buckley R.J., Kramm K., Cooper C.D.O., Grohmann D., Bolt E. L. (2020) Mechanistic insights into Lhr helicase function in DNA repair. Biochemical Journal. 477 (16): 2935-2947. DOI:10.1042/BCJ20200379 (external link, opens in a new window).

Kramm K., Schröder T., Gouge J., Vera A. M., Gupta K., Heiss F.B., Liedl T., Engel C., Berger I., Vannini A., Tinnefeld P. and Grohmann D. (2020). DNA origami-based single-molecule force spectroscopy elucidates RNA polymerase III pre-initiation complex stability.
Nature Communications 11: 2828. doi: 10.1038/s41467-020-16702-x. (external link, opens in a new window)

Liu L., Huber H., Berg I. A. (2020). Enzymes catalyzing crotonyl-CoA conversion to acetoacetyl-CoA during the autotrophic CO2 fixation in Metallosphaera sedula.
Front. Microbiol. 11; doi:10.3389/fmicb.2020.00354. (external link, opens in a new window)

Eder W., Tindall B. J., Huber H., Peplies J., Wirth R. (2020). Recognition of the unsuitability of DSM 12173 as the deposited type strain of Thermocrinis ruber Huber et al. 1999, recognition of DSM 23557 as an authentic sub-culture of strain OC 1/4, the nomenclatural type of Thermocrinis ruber Huber et al. 1999 and an emended description of Thermocrinis ruber Huber et al. 1999.
Archives of Microbiology. doi: 10.1007/s00203-020-01850-x. (external link, opens in a new window)

Eckl D. B., Huber H., Bäumler W. (2020). First report on Photodynamic Inactivation of Archaea including a novel method for high-throughput reduction measurement.
Photochemistry and Photobiology. doi: (external link, opens in a new window) 10. 1111/php.13229 (external link, opens in a new window).

Rose S., Auxilien S., Havelund J. F., Kirpekar F., Huber H., Grosjean H., Douthwaite S. (2020). The hyperthermophilic partners Nanoarchaeum and Ignicoccus stabilise their tRNA T-loops via different but structurally equivalent modifications.
Nucleic acids research. doi: (external link, opens in a new window) 10. 1093/nar/gkaa411 (external link, opens in a new window).

2019

S. Wu, Y. Zhou, D. Gerngross, M. Jeschek, T.R. Ward. (2019) Chemo-enzymatic cascades to produce cycloalkenes from bio-based resources.
Nature Communications 10:5060. https://www.nature.com/articles/s41467-019-13071-y (external link, opens in a new window)

Kramm K., Endesfelder U., Grohmann D. (2019). A single-molecule view of archaeal transcription.
Journal of Molecular Biology. doi: (external link, opens in a new window) 10. 1016/j.jmb.2019.06.009 (external link, opens in a new window).

Grünberger F., Reichelt R.M., Bunk B., Spröer C., Overmann J., Rachel R., Grohmann D., Hausner W. (2019) Next generation DNA-Seq and differential RNA-Seq allow re-annotation of the Pyrococcus furiosus DSM 3638 genome and provide insights into archaeal antisense transcription.
Frontiers in Microbiology 10, 1603. doi: 10.3389/fmicb.2019.01603. (external link, opens in a new window)

Kramm K., Engel C., Grohmann D. (2019) Transcription factor TBP: Old friend new questions.
Biochem Soc Trans. 47: 411 - 423. doi: (external link, opens in a new window) 10. 1042/BST20180623 (external link, opens in a new window).

Jakob L., Gust A., Grohmann D. (2019) Evaluation and optimisation of unnatural amino acid incorporation and bioorthogonal bioconjugation for site-specific fluorescent labelling of proteins expressed in mammalian cells.
Biochemistry and Biophysics Reports 17, 1-9. doi: (external link, opens in a new window) 10. 1016/j.bbrep.2018.10.011 (external link, opens in a new window).

Kreuter L. J., Weinfurtner A., Ziegler A., Weigl J., Hoffmann J., Morgner N. , Müller V., Huber H. (2019). Purification of a crenarchaeal ATP synthase in the light of the unique bioenergetics of Ignicoccus species.
J. Bacteriol. Vol 201, e00510-18; doi: (external link, opens in a new window) 10. 1128/JB.00510-18 (external link, opens in a new window).

Thema M., Weidlich T., Hörl M., Bellack A., Mörs F., Hackl F., Kohlmayer M., Gleich J., Stabenau C., Trabold T., Neubert M., Ortlo F., Brotsack R., Schmack D., Huber H., Hafenbradl D., Karl J., Sterner M. (2019).
Biological CO2-Methanation: An Approach to Standardisation. Energies 12, 1670; doi: (external link, opens in a new window) 10. 3390/en12091670 (external link, opens in a new window).

2018

T. Heinisch, F. Schwizer, B. Garabedian, E. Csibra, M. Jeschek, J. Vallapurackal, V.B. Pinheiro, P. Marlière, S. Panke, T.R. Ward. (2018). E. coli surface display of streptavidin for directed evolution of an allylic deallylase.
Chemical Science 9:5383-5388. https://pubs.rsc.org/en/content/articlelanding/2018/sc/c8sc00484f (external link, opens in a new window)

M. Jeschek, S. Panke, T.R. Ward. (2018) Artificial metalloenzymes on the verge of new-to-nature metabolism.
Trends in Biotechnology 36:60-72. https://pubs.rsc.org/en/content/articlelanding/2018/sc/c8sc00484f (external link, opens in a new window)

Reichelt R., Grohmann D., Willkomm S. (2018) A journey through the evolutionary diversification of archaeal Lsm and Hfq proteins.
Emerging topics in life sciences. 2: 647-657. doi: (external link, opens in a new window) 10. 1042/ETLS20180034 (external link, opens in a new window).

Dexl S., Reichelt R., Kraatz K., Schulz S., Grohmann D., Bartlett M., Thomm M. (2018) Displacement of the Transcription Factor B Reader Domain during Transcription Initiation.
Nucleic Acids Research. 46: 10066 - 10081. doi: 10.1093/nar/gky699. (external link, opens in a new window)

Molle J., Jakob L., Bohlen J., Raab M., Tinnefeld P., Grohmann D. (2018) Towards Structural Biology with Super-Resolution Microscopy.
Nanoscale. 10: 16416 - 16424. doi: (external link, opens in a new window) 10. 1039/c8nr03361g (external link, opens in a new window).

Willkomm S., Makaraova K. and Grohmann D., (2018) Prokaryotic Argonaute proteins represent an additional layer in prokaryotic defence.
FEMS Microbiology Reviews. 42:376-387. doi: 10.1093/femsre/fuy010. (external link, opens in a new window)

Gust A., Jakob L., Zeitler D., Willkomm S., Kramm K., Tinnefeld P., Meister G. and Grohmann D. (2018) Site-specific fluorescent labelling of endogenous mammalian proteins for single-molecule FRET analysis.
ChemBioChem. 19:780-783. doi: (external link, opens in a new window) 10. 1002/cbic.201700696. (external link, opens in a new window)

2017

M. Jeschek, M.O. Bahls, V. Schneider, P. Marlière, T.R. Ward, S. Panke. (2017). Biotin-independent strains of Escherichia coli for enhanced streptavidin production.
Metabolic Engineering 40:33-40. https://pubmed.ncbi.nlm.nih.gov/28062280/ (external link, opens in a new window)

Blombach F. and Grohmann D. (2017). Same same but different: the evolution of TBP in archaea and their eukaryotic offspring.
Transcription. 8:162-168. doi: 10.1080/21541264.2017.1289879. (external link, opens in a new window)

M. Jeschek, D. Gerngross, S. Panke. (2017) Combinatorial pathway optimisation for streamlined metabolic engineering.
Current Opinion in Biotechnology 47:142-151. https://pubmed.ncbi.nlm.nih.gov/28750202/ (external link, opens in a new window)

Gouge J., Guthertz N., Dergai O., Kramm K., Abascal-Palacios G., Satia K., Cousin P., Nouria Hernandez N., Grohmann D. and Vannini A. (2017). Molecular mechanisms of Bdp1 in TFIIIB assembly and RNA polymerase III transcription initiation.
Nature Structural and Molecular Biology, Nature Communications. 8(1): 130. doi: 10.1038/s41467-017-00126-1. (external link, opens in a new window)

Willkomm S., Zander A. and Grohmann D. (2017). Site-Specific Fluorescent Labelling of Argonaute for FRET-based Bio-Assays.
Methods in Molecular Biology, 1517:291-304. doi: (external link, opens in a new window) 10. 1007/978-1-4939-6563-2_20 (external link, opens in a new window).

Zander A., Willkomm S., Ofer S., van Wolferen M., Egert L., Buchmeier S., Stöckl S., Tinnefeld P., Schneider S., Klingl A., Albers S.-V., Werner F. and Grohmann D. (2016). Guide-independent DNA silencing by archaeal Argonaute.
Nature Microbiology. 2: 17034. doi: 10.1038/nmicrobiol.2017.35. (external link, opens in a new window)

Willkomm S, Oellig C. A., Zander A., Restle T., Keegan R., Grohmann D.* and Schneider, S. (2016). Structural and mechanistic insights into an archaeal DNA-guided Argonaute protein.
Nature Microbiology. 2:17035. doi: 10.1038/nmicrobiol.2017.35. (external link, opens in a new window)

Kucher S, Korneev S. Tyagi S., Apfelbaum R., Grohmann D., Lemke E.A., Klare, J., Steinhoff H.-J., Klose, D. (2016) Orthogonal spin labelling using click chemistry for in vitro and in vivo applications.
Journal of Magnetic Resonance, 275:38-45. doi: (external link, opens in a new window) 10. 1016/j.jmr.2016.12.001 (external link, opens in a new window).

2016

M. Jeschek, S. Panke, T. R. Ward. (2016). Periplasmic screening for artificial metalloenzymes.
Methods in Enzymology 580:539-556. https://pubmed.ncbi.nlm.nih.gov/27586348/ (external link, opens in a new window)

M. Jeschek, R. Reuter, T. Heinisch, C. Trindler, J. Klehr, S. Panke, T.R. Ward. (2016). Directed evolution of artificial metalloenzymes for in vivo metathesis.
Nature 537:661-665. https://www.nature.com/articles/nature19114 (external link, opens in a new window)

M. Jeschek, D. Gerngross, S. Panke. (2016) Rationally reduced libraries for combinatorial pathway optimisation minimising experimental effort.
Nature Communications 7:11163. https://www.nature.com/articles/ncomms11163 (external link, opens in a new window)

Sheppard C., Blombach F., Belsom A., Schulz S., Daviter T., Smollett K., Mahieu E., Erdmann S., Tinnefeld P., Garrett R., Grohmann D., Rappsilber J., Werner F. (2016) Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP.
Nature Communications, 7:13595. doi: (external link, opens in a new window) 10. 1038/ncomms13595 (external link, opens in a new window).

Nickels P.C., Wünsch B., Holzmeister P., Bae W., Kneer L.M., Grohmann D., Tinnefeld P. and Liedl T. (2016), Molecular Force Spectroscopy with a DNA Origami-Based Nanoscopic Force Clamp.
Science. 354(6310):305-307. doi: (external link, opens in a new window) 10. 1126/science.aah5974 (external link, opens in a new window).

Willkomm S., Zander A., Grohmann D. and Restle T. (2016). Mechanistic insights into archaeal and human Argonaute substrate binding and cleavage properties.
PLOS one, 11(10):e0164695. doi: (external link, opens in a new window) 10. 1371/journal.pone.0164695 (external link, opens in a new window).

Blombach B., Smollett K.L., Grohmann D. and Werner F. (2016). Molecular Mechanisms of Transcription Initiation - structure, function and evolution of TFE/TFIIE-like factors and open complex formation.
Journal of Molecular Biology (epub, ahead of print). doi: (external link, opens in a new window) 10. 1016/j.jmb.2016.04.016 (external link, opens in a new window).

Schulz S., Gietl A., Smollett K., Tinnefeld P., Werner F. and Grohmann D. (2016). TFE and Spt4/5 open and close the RNA polymerase clamp during the transcription cycle.
PNAS (epub, ahead of print) Selected by the Faculty 1000

Jakob L., Treiber T., Treiber N., Gust A., Kramm K., Hansen K., Stotz M., Wankerl L., Herzog F., Hannus S., Grohmann D., and Meister G. (2016). Structural and functional insights into the fly microRNA biogenesis factor Loquacious.
RNA. (epub ahead of print). doi: (external link, opens in a new window) 10. 1261/rna.055426.115 (external link, opens in a new window).

Molle J., Raab M., Holzmeister S., Schmitt-Monreal D., Grohmann D., He Z., Tinnefeld P. (2016). Superresolution microscopy with transient binding.
Current Opinion in Biotechnology, 39: 8-16. doi: 10.1016/j.copbio.2015.12.009. (external link, opens in a new window)

2015

Schulz S., Kramm K., Werner F., Grohmann D. (2015). A fluorescently labelled recombinant RNAP system to probe archaeal transcription initiation.
Methods (epub, ehead of print). doi: (external link, opens in a new window) 10. 1016/j.ymeth.2015.04.017 (external link, opens in a new window).

Willkomm S., Zander A., Gust A., Grohmann D. (2015). A prokaryotic twist on Argonaute action.
Life. 5 (1), 538-553. doi: (external link, opens in a new window) 10. 3390/life5010538 (external link, opens in a new window).

Nagy J., Grohmann D., Cheung A.C.M., Schulz S., Smollett K., Werner F. & Michaelis J. (2015): Complete architecture of the archaeal RNA polymerase open complex from single-molecule FRET and NPS.
Nature Communications, 6:6161. doi: 10.1038/ncomms7161 (external link, opens in a new window)

2014

A. Gust, A. Zander, A. Gietl, P. Holzmeister, S. Schulz, B. Lalkens, P. Tinnefeld, D. Grohmann (2014): A Starting Point for Fluorescence-Based Single-Molecule Measurements in Biomolecular Research.
Molecules, 19, 10, 15824-15865. doi: (external link, opens in a new window) 10. 3390/molecules191015824 (external link, opens in a new window).

Acuna G., Grohmann D., Tinnefeld P. (2014) Enhancing single-molecule fluorescence with nanophotonics.
FEBS letters. doi: 10.1016/j.febslet.2014.06.016 (external link, opens in a new window)

Gietl A., Holzmeister P., Blombach F., Schulz S., Voithenberg L.V.v., Lamb D., Werner F., Tinnefeld P., Grohmann D. (2014) Eukaryotic and Archaeal TBP and TFB/TF(II)B Follow Different Promoter DNA Bending Pathways.
Nucleic Acids Research. 42(10):6219-31. Selected by the Faculty 1000. doi: (external link, opens in a new window) 10. 1093/nar/gku273 (external link, opens in a new window).

Zander A., Holzmeister P., Klose D., Tinnefeld P. and Grohmann D. (2014). Single-molecule FRET supports the two-state model of Argonaute action.
RNA Biology 11(1):45-56. (featured image from our article). doi: (external link, opens in a new window) 10. 4161/rna.27446 (external link, opens in a new window).

Holzmeister P., Acuna G.P., Grohmann D. and Tinnefeld P. (2014) Breaking the Concentration Limit of Optical Single-Molecule Detection.
Chemical Society Reviews 43(4):1014-28. (back cover). doi: (external link, opens in a new window) 10. 1039/C3CS60207A (external link, opens in a new window).

Afanasyeva A., Hirtreiter A., Schreiber A., Grohmann D., Pobegalov E., McKay A.R., Tsaneva I., Petukhov M., Käs E., Grigoriev M. and Werner F. (2014). Lytic water dynamics reveal evolutionarily conserved mechanisms of ATP hydrolysis by TIP49 AAA+ ATPases.
Structure. 22(4):549-59. doi: (external link, opens in a new window) 10. 1016/j.str.2014.02.002 (external link, opens in a new window).

2013

Grohmann D.*, Werner F. and Tinnefeld P. (2013). Making Connections - Strategies for Single Molecule Fluorescence Biophysics.
Current Opinion in Chemical Biology. 17(4):691-8. doi: (external link, opens in a new window) 10. 1016/j.cbpa.2013.05.020 (external link, opens in a new window).

Gietl A. and Grohmann D. (2012). Modern biophysical approaches probe transcription factor induced DNA bending and looping.
Biochem Soc Trans. 41(1):368-73. doi: (external link, opens in a new window) 10. 1042/BST20120301 (external link, opens in a new window).

2012

Blombach F., Daviter T., Fielden D., Grohmann D., Smollett K. and Werner F. (2012). Archaeology of RNA polymerase - factor swapping during the transcription cycle.
Biochem Soc Trans. 41(1):362-7. doi: 10.1038/nmicrobiol.2017.21. (external link, opens in a new window)

Klose D., Klare J., Grohmann D., Kay C.W.M., Werner F. and Steinhoff H-J. (2012). Simulation vs. reality: a comparison of in silico predictions with DEER and FRET measurements.
PLOS one, 7(6):e39492. doi: (external link, opens in a new window) 10. 1371/journal.pone.0039492 (external link, opens in a new window).

Gietl A., Holzmeister P., Grohmann D.* and Tinnefeld P. (2012). DNA origami as biocompatible surface to match single-molecule and ensemble experiments.
Nucleic Acids Research, epub. doi: (external link, opens in a new window) 10. 1093/nar/gks326 (external link, opens in a new window).

2011

Grohmann D., Nagy J., Chakraborty A., Klose D., Fielden D., Ebright R.H., Michaelis J., Werner F. (2011). The Initiation Factor TFE and the Elongation Factor Spt4/5 Compete for the RNAP Clamp during Transcription Initiation and Elongation.
Molecular Cell, 43:263-74. doi: (external link, opens in a new window) 10. 1016/j.molcel.2011.05.030 (external link, opens in a new window).

Grohmann D. and Werner F. (2011) Recent advances in the understanding of archaeal transcription.
Curr Opin Microbiol. doi: (external link, opens in a new window) 10. 1016/j.mib.2011.04.012 (external link, opens in a new window).

Ryan C.P., Smith M.E., Schumacher F.F., Grohmann D., Papaioannou D., Waksman G., Werner F., Baker J.R., Caddick S. (2011) Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis.
Chem Commun. 47:5452-4. doi: (external link, opens in a new window) 10. 1039/c1cc11114k (external link, opens in a new window).

Grohmann D., Klose D., Fielden D., Werner F. (2011) FRET (fluorescence resonance energy transfer) sheds light on transcription.
Biochem Soc Trans. 39:122-7. doi: (external link, opens in a new window) 10. 1042/BST0390122 (external link, opens in a new window).

Werner F. and Grohmann D. (2011) Evolution of multisubunit RNA polymerases in the three domains of life.
Nature Reviews Microbiol.9:85-98. doi: 10.1038/nrmicro2507. (external link, opens in a new window)

Grohmann D. and Werner F. (2011) Cycling through Transcription with the RNA polymerase F/E (RPB4/7) Complex - Structure, Function and Evolution of Archaeal RNA polymerase.
Research in Microbiology 162:10-8. doi: 10.1016/j.resmic.2010.09.002. (external link, opens in a new window)

2010

Grohmann D., Klose D., Klare J.P., Kay C.W., Steinhoff H.J., Werner F. (2010) RNA-binding to archaeal RNA polymerase subunits F/E: a DEER and FRET study.
J Am Chem Soc, 132:5954-5. doi: (external link, opens in a new window) 10. 1021/ja101663d (external link, opens in a new window).

Hirtreiter A., Damsma G.E., Cheung A.C., Klose D., Grohmann D., Vojnic E., Martin A.C., Cramer P., Werner F. (2010). Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif.
Nucleic Acids Research 38:4040-51. doi: 10.1093/nar/gkq135. (external link, opens in a new window)

Hirtreiter A., Grohmann D., Werner F. (2010) Molecular mechanisms of RNA polymerase - the F/E (Rpb4/7) complex is required for high processivity in vitro.
Nucleic Acids Research, 38:585-96. doi: 10.1093/nar/gkp928. (external link, opens in a new window)

Grohmann, D. & Werner, F. (2010) Hold on!: RNA polymerase interactions with the nascent RNA modulate transcription elongation and termination.
RNA Biology, 7: 310-315. doi: 10.4161/rna.7.3.11912. (external link, opens in a new window)

2009

Grohmann D., Hirtreiter A., Werner F. (2009). RNAP subunits F/E (Rpb4/7) are stably associated with archaeal RNA polymerase: using fluorescence anisotropy to monitor RNAP assembly in vitro.
Biochem J, 421:339-43. (selected by the Faculty 1000). doi: (external link, opens in a new window) 10. 1042/BJ20090782 (external link, opens in a new window).

Grohmann D., Hirtreiter A., Werner F. (2009), Molecular mechanisms of archaeal RNA polymerase.
Biochem Soc Trans. 37:12-7. doi: 10.1042/BST0370012. (external link, opens in a new window)

2008

Grohmann D., Godet J., Mely Y., Darlix JL. and Restle, T. (2008). HIV-1 NC traps the reverse transcriptase on primer/template substrates.
Biochemistry, 47:12230-40. doi: (external link, opens in a new window) 10. 1021/bi801386r (external link, opens in a new window).

Di Pasquale F, Fischer D, Grohmann D, Restle T, Geyer A, Marx A. (2008) Opposed steric constraints in human DNA polymerase beta and E. coli DNA polymerase I.
J Am Chem Soc 130:10748-57. doi: (external link, opens in a new window) 10. 1021/ja8028284 (external link, opens in a new window).

Grohmann D., Corradi V., Horenkamp F., Laufer, S.D., Manetti, F. Botta, M. and Restle, T. (2008). Small molecule inhibitors targeting HIV-1 dimerisation.
Chembiochem 9:916-22. doi: 10.1002/cbic.200700669. (external link, opens in a new window)

[Translate to English:] 2007

Yamazaki S., Tan L., Mayer G., Hartig J.S., Song J.N., Reuter S., Restle T., Laufer S.D., Grohmann D., Kräusslich H.G., Bajorath J., Famulok M. (2007). Aptamer displacement identifies alternative small-molecule target sites that escape viral resistance.
Chem Biol.14:804-12. doi: 10.1016/j.chembiol.2007.06.003. (external link, opens in a new window)

[Translate to English:] 2006

Mescalchin A., Wünsche W., Laufer S.D., Grohmann, D., Restle, T. and Sczakiel, G. (2006). Specific binding of a bioactive hexanucleotide to HIV-1 reverse transcriptase: a novel class of small oligomeric nucleic acid drugs.
Nucleic Acids Research, 34, 5631-5637. doi: (external link, opens in a new window) 10. 1093/nar/gkl533 (external link, opens in a new window).