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Kompetenzzentrum Fluoreszente Bioanalytik

Kfb Staff

The Kompetenzzentrum Fluoreszente Bioanalytik (KFB) is the Genomics Core Unit (GCU) of the University of Regensburg. The KFB supports state-of-the-art genomics services for the local research community, as well as for external partners and clients across Europe.

Our facility has contributed to hundreds of publications in peer-reviewed journals. Click here for an up-to-date list.

Our lab is equipped with the following platform technologies:

  • Deep Sequencing (Illumina NextSeq 2000; Illumina NextSeq 500/550): a growing range of sequencing applications and protocols are supported, including mRNA-Seq, total RNA-Seq, gDNA-Seq, ChIP-Seq, smallRNA-Seq.
  • Affymetrix GeneChip Microarrays (Thermo Fisher): RNA expression profiling using single-cartridge arrays (GeneChip Scanner 3000).

 
In addition, we offer stand-alone access to the following instruments:          
 

  • StepOne Plus Real-Time PCR System (Thermo Fisher)
  • Agilent Bioanalyzer 2100 (DNA / RNA QC)
  • Covaris S2 AFA (highly-reproducible DNA fragmentation; recommended in many different sequencing library prep protocols)
  • Precellys 24 Homogenisator (bead-based disruption/homogenization of a wide range of tissues)
  • Nanodrop 1000 Spectrophotometer (DNA / RNA quantification)

Our experienced staff provides support along the full experimental workflow: e. g. project design/counseling, cost estimation/preparation of quotes, choice of kits and protocols, sample QC, sample preparation, data QC, and data analysis.

KFB is a member of the SFB960  and a cooperation partner of the Regensburger Centrum für Interventionelle Immunologie (RCI).

Sample submission:

Please bring in samples, or send them on dry ice. Don’t forget to fill in and sign the corresponding order form:

Illumina NextSeq deep sequencing service (english / german)

Affymetrix (Thermo Fisher) GeneChip microarray service (english / german)

Pricing:
Please contact us (Email: info@kfb-regensburg.de) for pricing, as charges vary per institutional affiliation, and depending on the payment model (fee-for-service, or cooperative).

Acknowledgements:

Researchers who use our Affymetrix microarray service on a fee-for-service basis must acknowledge KFB as follows: “Sample processing and Affymetrix microarray hybridization were carried out at the Genomics Core Unit: Center of Excellence for Fluorescent Bioanalytics (KFB, University of Regensburg, Germany).”

Researchers who use our Illumina Deep Sequencing services on a fee-for-service basis must acknowledge KFB as follows: “Illumina deep sequencing was carried out at the Genomics Core Unit: Center of Excellence for Fluorescent Bioanalytics (KFB, University of Regensburg, Germany).”

Terms of use:
Please download our Nutzungsordnung (.pdf).


  1. STARTSEITE UR

Kompetenzzentrum Fluoreszente Bioanalytik

Dr. Thomas Stempfl

 

Thomas Stempfl

 

Tel. 0941/943-5011
Fax: 0941/943-5018
info@kfb-regensburg.de