Chromatin remodelers are key players in the regulation of chromatin accessibility and nucleosome positioning on the eukaryotic DNA, therefore they are essential for all DNA dependent biological processes. There are mounting evidence that variety of cancers employ chromatin remodelers: they can be downregulated or even lacking functional protein, overexpressed, carry somatic mutations or support cancer through the interaction with long noncoding RNA. In parallel, the promise of epigenetic therapies is growing. It seems that targeting and exploring the pharmaceutical potential of deregulated chromatin remodeling machines might lead to more efficient personalized cancer therapies.
We are studying chromatin remodeling complexes and trying to further understand the mechanisms of how chromatin remodelers act in cancer using a combination of biochemical, molecular biology, genetic and mamalian cell culture techniques. In collaboration with 2bind company we are characterizing the cancer occurring mutations in chromatin remodelers.
Laura Manelyte - Short CV
|Principal Investigator (DFG funded) at the University of Regensburg, Department of Biochemistry III|
|2014 - 2015||Postdoctoral researcher (Stipend, from Bavarian Ministry "Programm zur Realisierung der Chancengleichheit für Frauen in Forschung und Lehre") at the University of Regensburg, Department of Biochemistry III (Prof. G. Längst)|
|2009 - 2013|| |
Postdoctoral researcher at the University of Regensburg (Germany), Department of Biochemistry III (Prof. G. Längst)
|Postdoctoral researcher at the University of Bristol (UK), School of Biochemistry (Prof. N.Savery)|
|2003 - |
|Doctorate (Dr.rer.nat, summa cum laude) at the Justus-Liebig University Giessen (Germany), Department of Biochemistry (Prof. P.Friedhoff and Prof. A.Pingoud)|
Manelyte L (2017) Chromatin remodelers, their implication in cancer and therapeutic potential J Rare Dis Res Treat. (2017) 2(3): 34-40.
Friedhoff P, Manelyte L, Giron-Monzon L, Winkler I, Groothuizen FS, Sixma TK (2017) Use of Single-Cysteine Variants for Trapping Transient States in DANN Mismatch Repair Methods Enzymol. 2017;592:77-101.
Längst G* and Manelyte L* (2015) Chromatin remodelers: from function to dysfunction Genes Jun 12;6(2):299-324.
Manelyte L, Strohner R, Gross T and Längst G (2014) Chromatin targeting signals, nucleosome positioning mechanism and non-coding RNA-mediated regulation of the chromatin remodeling complex NoRC, Plos Genetics. 2014 Mar 20;10(3):e1004157.
Manelyte L and Längst G (2013) Chromatin remodelers and their way of action (Chromatin remodelling book) InTechOpen.
Groothuizen FS, Fish A, Petoukhov MV, Reumer A, Manelyte L, Winterwerp HH, Marinus MG, Lebbink JH, Svergun DI, Friedhoff P, Sixma TK (2013) Using stable MutS dimers and tetramers to quantitatively analyze DNA mismatch recognition and sliding clamp formation, Nucleic Acids Res., Sep;41(17):8166-81
Winkler I, Marx AD, Lariviere D, Manelyte L, Giron-Monzon L, Heinze RJ, Cristovao M, Reumer A, Curth U, Sixma TK and Friedhoff P (2011) Chemical trapping of the dynamic MutS-MutL complex formed in DNA mismatch repair in Escherichia coli. J.Biol.Chem., 286: 22706.
Manelyte L, Kim YI, Smith AJ, Smith RM and Savery NJ (2010) Regulation and rate-enhancement during transcription-coupled DNA repair. Mol Cell, 40(5):714-24.
Manelyte L, Guy CP, Smith RM, McGlynn P, Dillingham M and Savery NJ (2009) The unstructured C-terminal extension of UvrD interacts with UvrB, but is dispensable for nucleotide excision repair, DNA Repair 8(11):1300-10.
Murphy MN, Peng G, Kenneth R, Manelyte L, Savery NJ and Karsten T (2009) An N-terminal clamp restrains the motor domains of the bacterial transcription-repair coupling factor Mfd, Nucleic Acids Res. (18):6042-53
Manelyte L, Urbanke C, Giron-Monzon L, Friedhoff P (2006) Structural and functional analysis of the MutS C-terminal tetramerization domain, Nucleic Acids Res., 34(18):5270-9.
Ahrends R, Kosinski J, Kirsch D, Manelyte L, Giron-Monzon L, Hummerich L, Schulz O, Spengler B, Friedhoff P (2006) Identifying an interaction site between MutH and the C-terminal domain of MutL by crosslinking, affinity purification, chemical coding and mass spectrometry, Nucleic Acids Res., 34(10):3169-80.
Giron-Monzon L, Manelyte L, Ahrends R, Kirsch D, Spengler B, Friedhoff P (2004), Mapping protein-protein interactions between MutL and MutH by cross-linking, J.Biol.Chem., 19;279(47):49338-45.
Daujotyte D, Vilkaitis G, Manelyte L, Skalicky J, Szyperski T, Klimasauskas S (2003), Solubility engineering of the HhaI methyltransferase, Protein Eng., 16(4):295-301.
Dr. Laura Manelyte
Department of Biochemistry III
Biochemistry Center Regensburg
University of Regensburg
Room PHY 8.0.22
T: +49 941 943 2867