Zu Hauptinhalt springen
Startseite UR

Research

Research interests

 

  • Physiology and patho-physiology of electrolyte transport in kidney, respiratory tract, and intestine.

  • Patho-physiology and molecular biology of  polycystic kidney disease.

  •  Patho-physiology and molecular biology of cystic fibrosis and other inflammatory airway diseases.

  • Development of novel ways for the treatment of asthma and cystic fibrosis lung disease. Exploring the possibility of targeting alternative ion channels.

  • Role of ion channels for cell proliferation and cancer.

  •  Role of ion channels in inflammatory diseases.

  •  Contribution of TMEM16 proteins to regulated cell death, e.g. ferroptosis, apoptosis, and pyroptosis.

 

Methods used

      Electrophysiology

                             

-          Open and short circuit measurements in Ussing chambers

-          Patch clamp techniques in all applications

                                                               

     

      Fluorescence based techniques

                             

-          Immunostaining of cellular components, soluble and membrane proteins in       Epithelial and non-epithelial cells

-          Fluorescence ratio measurements of intracellular pH, Ca2+ and Cl-

-          Confocal microscopy and video imaging

-          Iodide quenching of YFP fluorescence in plate reader and single cell microscopy

                                        

 

      Cell biological techniques

                             

-          Primary and permanent cell culture of epithelial and non-epithelial cells

-          S2 techniques including viral transfection, infectious agents

-          Cell tracking by HoloMonitor M4 Microscopy

-          Casy flow cytometry

-          Accury flow cytometry 4 wavelengths

-          Reconstitution of membrane proteins in liposomes

                                  

                                                     

      Protein biochemistry & Molecular biology

                             

-          Western blotting

-          Immunoprecipitation, coimmunoprecipitation

-          DNA-cloning techniques

-          site directed mutagenesis

-          PCR - applications

-          DNA sequencing

-          siRNA techniques

-          CRISPR-Cas technology

 

      Knockout technology

                             

-          In vivo and ex vivo animals studies

-          Conventional and Cre/loxP knockout mice

-          Inducible Cre/loxP knockout mice

 

 

 


  1. Fakultät für Biologie und Vorklinische Medizin
  2. INSTITUT FÜR PHYSIOLOGIE

Prof. Dr. med. Karl Kunzelmann


Sekretariat:
Hildegard Westermeier
Raum VKL4.1.30
Tel.: 0941 943-2947
Fax: 0941 943-4315
Hildegard.Westermeier@ur.de
Mo - Fr von 8-12 Uhr