Genetics - Universität Regensburg

Focus Subject - Genetics

You have to choose the theoretical module and one practical module.

TM Theoretical Module (12 CP)

Genetics

Prof. Dr. Wolfgang Seufert, Prof. Dr. Frank Sprenger, PD Jan Medenbach

PM Practical Modules (12 CP each)

Regulation of Cell Division and mRNA Translation	Prof. Dr. Wolfgang Seufert
Regulation of Cell Division in Drosophila	Prof. Dr. Frank Sprenger
Post-transcriptional regulation of gene expression	PD Dr. Jan Medenbach

Registration

Please contact Prof. Seufert (wolfgang.seufert@ur.de), Prof. Sprenger (frank.sprenger@ur.de) or PD Dr. Jan Medenbach (Jan.Medenbach@vkl.uni-regensburg.de), respectively, for an application to the practical modules of the Focus Subject (see also the infos to the practical modules). Information about the organization of the module, the times of the lecture (LSF-P-(D)-54402) and the seminar (LSF-P-(D)-54428) associated with the module, see this GRIPS course (46243). Usually, we have more students interested in the practical modules than our capacity allows. Based on the applications (see the info in the GRIPS course), participants for the individual practical modules will be allocated to avaiable time slots during the WS20/21 and SS21. Unfortunately, restrictions imposed by the corona situation limit our capacity additionally.

TM Genetics

BIO-M-TM-G

Detailed module description - pdf

Prof. Dr. Wolfgang Seufert, Prof. Dr. Frank Sprenger, PD Dr. Jan Medenbach

Module contents

Cell Cycle: common regulatory mechanisms in the control of the cell cycle, DNA-replication, DNA-damage response, mitosis, meiosis, cytokinesis.
Translation: Regulation of translation of eucaryotic mRNAs
Presentation and discussion of a research based topic from the topics listed above
Elaboration and presentation of methods that are used in cell cycle and translational control

Module components

Lecture: Cell Cycle Control in Eucaryotes / Control of Cell Proliferation (LSF-No. P-(D)-54402)
Lecture: A lecture in the field of genetics or molecular or cellular biology. Please select a lecture (see LSF for list of lectures) and confirm with your oral examiner if this lecture would be suitable.
Seminar: Seminar on Current Topics in Cell Cycle and Translation Control / Cell Proliferation Control (LSF-No. P-(D)-54428)

In the seminar No. 3 a seminar presentation has to be held and registered in Flexnow as an ungraded study achievement (Studienleistung).

Module examination

30 min oral examination (graded)

The student can choose the examiner.

(Der Prüfer kann vom Studierenden frei gewählt werden.)

PM Regulation of Cell Division and mRNA Translation

BIO-M-PM-CDT

Detailed module description - pdf

Prof. Dr. Wolfgang Seufert (link to research group)

Application for practical Module in the lab of Wolfgang Seufert for WS19/20 and SS 20: (Application form will be posted shortly)

Application for practical Module in the lab of Jan Medenbach for WS19/20 and SS 20: Please downlods this form and send it back to Jan Medenbach

Module contents

Analysis of the cell division cycle in budding yeast by use of classical genetic techniques such as mating, sporulation and tetrade dissection as well as cell synchronization, confocal fluorescence microscopy, time-lapse live-cell imaging, flow cytometry and further methods
Analysis of the assembly of eIF2 by Cdc123 using PCR-mediated epitope-tagging, Western analysis, co-immunoprecipitation, yeast two-hybrid assays, heterologous expression and affinity-purification of proteins, ThermoFluor analysis and further methods
Analysis of the control of mRNA translation by gene reporter assays, RNA preparation, RT-qPCR and RNAseq analysis, polysome profiling and further methods
Presentation and discussion of own experimental gained data

Module components

Laboratory course (6 weeks) with seminar: Regulation of Cell Division and mRNA Translation

Module examination

Portfolio examination (graded)

PM Regulation of Cell Division in Drosophila

BIO-M-PM-CDD

Detailed module description - pdf

Prof. Dr. Frank Sprenger (link to research group)

Application for WS19/20 and SS 20: Please downlods this form and send it back to Prof.Sprenger

Module contents

In a six-week research project the student will work under guidance on a current research topics in the field of cell cycle regulatory processes. He will use, among other things, tissue culture cells (from Drosophila and vertebrates) as well as different developmental stages from Drosophila.

The student will present and discuss the data obtained in the research project as well as the relevant scientific background in a written report and will present and discuss the results in an oral presentation.

Module components

Laboratory course (6 weeks) with seminar: Regulation of Cell Division in Drosophila

Module examination

Portfolio examination (graded)

PM Post-transcriptional regulation of gene expression

BIO-M-PM-PTR

Detailed module description - pdf

PD Dr. Jan Medenbach (link to research group)

Module contents

Cell-biological and biochemical experimentation aimed to analyse post-transcriptional regulation of gene expression
Eukaryotic tissue culture: maintenance and transfection of eukaryotic cells, preparation of cellular extracts, isolation of nucleic acids
Reporter gene analyses: e.g. cloning of reporter genes, RT-PCR analyses of reporter genes, luciferase assays, and/or analysis of gene expression by Western blotting
Expression and purification of recombinant proteins
Presentation and discussion of experimental data obtained during the practical course (during the lab seminar)

Module components

Laboratory course (6 weeks) with seminar: Post-transcriptional regulation of gene expression

Module examination

Portfolio examination (graded)