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Biochemistry

Focus Subject - Biochemistry

You have to choose one theoretical module and one practical module.

Theoretical Modules (TM)

Protein Biochemistry and Enzymology Prof. Dr. Reinhard Sterner
RNA Biochemistry Prof. Dr. Gunter Meister

Practical Modules (PM)

Protein Biochemistry and Enzymology Prof. Dr. Reinhard Sterner
RNA Biology Prof. Dr. Gunter Meister
Chromatin Dynamics and Nuclear Architecture Prof. Dr. Gernot Längst
Ribosome Biogenesis Prof. Dr. Herbert Tschochner
Post-transcriptional regulation of gene expression PD Dr. Jan Medenbach

TM Protein Biochemistry and Enzymology

BIO-M-TM-PBE

Detailed module description - pdf

Prof. Dr. Reinhard Sterner

It is recommended to simultaneously attend the practical module BIO-M-PM-PBE Protein Biochemistry and Enzymology.

Module contents
Lectures
  • Methods of physical biochemistry: e.g. optical spectroscopy, hydrodynamics, chromatography, electrophoresis
  • Structure and stability of biomolecules: e.g. protein folding, protein stability, nucleic acid-protein interactions
Seminar
  • Presentation and discussion of a research based topic in the field of protein biochemistry and enzymology
Module components
  1. at least two lectures in the field of protein biochemistry and enzymology (for a selection see LSF)
  2. one seminar in the field of protein biochemistry and enzymology (for a selection see LSF)

In the seminar a seminar presentation has to be held and registered in Flexnow as an ungraded study achievement (Studienleistung).

Module examination

30 min oral examination (graded)


TM RNA Biochemistry

BIO-M-TM-RNA

Detailed module description - pdf

Prof. Dr. Gunter Meister (link to research group)

Module contents
Lectures
  • Discussion of the biogenesis and the diverse functions of coding and non-coding RNAs
  • Introduction of the different classes of regulatory non-coding RNAs
  • Mediation of basic knowledge in RNA-RNA, RNA-DNA and RNA-Protein interactions
  • Presentation of current research directions in the field of RNA biochemistry/biology
  • Insights into current methodical approaches and applications of RNA biochemistry
  • Illustration of the role of RNA in disease and its potential therapeutic applications
Seminar
  • Presentation and discussion of a current research-based topic in the field of RNA biochemistry/biology
Module components
  1. at least two lectures in the field of RNA biochemistry (for a selection see LSF)
  2. one seminar in the field of RNA biochemistry (for a selection see LSF)

In the seminar a seminar presentation has to be held and registered in Flexnow as an ungraded study achievement (Studienleistung).

Module examination

30 min oral examination (graded)


PM Protein Biochemistry and Enzymology

BIO-M-PM-PBE

Detailed module description - pdf

Prof. Dr. Reinhard Sterner (link to research group)

It is recommended to simultaneously attend the theoretical module BIO-M-TM-PBE Protein Biochemistry and Enzymology.

Module contents
  • Participation in a current research project of protein biochemistry
  • Training in planning, conducting and evaluating experiments
  • Training in search of relevant literature
  • Getting to know and using modern methods of protein biochemistry and enzymology
  • Presentation and discussion of own experimentally gained data
Module components

Laboratory course (6 weeks) with seminar: Protein Biochemistry and Enzymology

Module examination

Portfolio examination (graded)


PM RNA Biology

BIO-M-PM-RNA

Detailed module description - pdf

Prof. Dr. Gunter Meister (link to research group)

Module contents
  • Introduction into a current research field of the group
  • Mediation of basic molecular biological and biochemical applications
  • Consolidation of current RNA biochemical methods corresponding to the research project
  • Independent theoretical processing of the whole project
  • Presentation and discussion of own experimental data in a written and an oral presentation
Module components

Laboratory course (6 weeks) with seminar: RNA Biology

Module examination

Portfolio examination (graded)


PM Chromatin Dynamics and Nuclear Architecture

BIO-M-PM-CD

Detailed module description - pdf

Prof. Dr. Gernot Längst (link to research group)

Module contents
  • Functional analysis of chromatin modifying enzymes in vitro and in vivo.
  • Characterisation of the chromatin structures and dynamic mechanisms in human and murine cells and as well in the Malaria causing parasite Plasmodium falciparum.
  • Determining the mechanism of sequence specific DNA access in chromatin and characterisation of the genome architecture by high throughput sequencing methods and bioinformatics.
  • Functional characterisation of non-coding RNA binding to chromatin.
  • Sequence specific binding of non-coding RNA to DNA via the formation of triple helices.
  • Quantification of molecular interactions by the microscale thermophoresis method.
  • Presentation and discussion of own experimental gained data.
Module components

Laboratory course (6 weeks) with seminar: Chromatin Dynamics and Nuclear Architecture

Module examination

Portfolio examination (graded)


PM Ribosome Biogenesis

BIO-M-PM-RB

Detailed module description - pdf

Prof. Dr. Herbert Tschochner (link to research group)

Module contents
  • In vivo, ex vivo and in vitro experiments to study ribosome biogenesis in the model organism S. cerevisiae and in archaea, for example
  • Analysis of the RNA polymerase I transcription machinery
  • Analysis of rDNA chromatin
  • Assembly of pre-ribosomes
  • Possibilities exist to use cellbiological, genetic and biochemical approaches to analyse synthesis and processing of RNA, structure and function of chromatin as well as mass spectrometric proteom analyses
  • Presentation and discussion of own experimental gained data
Module components

Laboratory course (6 weeks) with seminar: Ribosome Biogenesis

Module examination

Portfolio examination (graded)


PM Post-transcriptional regulation of gene expression

BIO-M-PM-PTR

Detailed module description - pdf

PD Dr. Jan Medenbach  (link to research group)

Module contents
  • Cell-biological and biochemical experimentation aimed to analyse post-transcriptional regulation of gene expression
  • Eukaryotic tissue culture: maintenance and transfection of eukaryotic cells, preparation of cellular extracts, isolation of nucleic acids
  • Reporter gene analyses: e.g. cloning of reporter genes, RT-PCR analyses of reporter genes, luciferase assays, and/or analysis of gene expression by Western blotting
  • Expression and purification of recombinant proteins
  • Presentation and discussion of experimental data obtained during the practical course (during the lab seminar)
Module components

Laboratory course (6 weeks) with seminar: Post-transcriptional regulation of gene expression

Module examination

Portfolio examination (graded)

  1. Fakultät für Biologie und Vorklinische Medizin
  2. Studium