Our platform technologies include lateral-flow assays, paper-based microfluidic assays, and miniaturized microfluidic systems as lab-on-a-chip devices. Signal amplification strategies are central to our sensor development and embrace a variety of different approaches including investigations toward highly sensitive detection strategies using electrochemical methods (such as with laser-induced graphene electrodes), electrochemiluminescence (enabled through liposomes), and luminescence (externer Link, öffnet neues Fenster), as well as new materials such as upconverting nanoparticles, 2D nanomaterials and nanofibers. Hence, our several technologies are integrated and synergistically form those on-site and high-throughput systems that allow quantitative detection of complex analytes in their original samples. We focus on multi-analyte detection of pathogens, toxins, cells and metabolites ranging from protozoan parasites like Cryptosporidium parvum to the blood clotting protein thrombin.
For example:
- Bauer, M., Duerkop, A., Baeumner, A.J. “Critical review of polymer and hydrogel deposition methods for optical and electrochemical bioanalytical sensors correlated to the sensor’s applicability in real samples.” Analytical and Bioanalytical Chemistry, (2022) 415, 83 – 95, https://doi.org/10.1007/s00216-022-04363-2
- Perju, A.T., Baeumner, A.J., Wongkaew, N., “Freestanding 3D-interconnected carbon nanofibers as high-performance transducers in miniaturized electrochemical sensors” Microchimica Acta (2022), 189, 424, https://doi.org/10.1007/s00604-022-05492-2
- Gerstl, F., Pongkitdachoti. U., Unob, F. and Baeumner, A.J. “Miniaturized Sensor for Electroanalytical and Electrochemiluminescent Detection of Pathogens enabled through Laser-Induced Graphene Electrodes embedded in Microfluidic Channels” Lab on a Chip (2022) 22, 3721 – 3733, https://doi.org/10.1039/D2LC00593J
- Beck, F., Horn, C., Baeumner, A.J. “Ag Nanoparticles Outperform Au Nanoparticles for the Use as Label in Electrochemical Point-of-Care Sensors” Analytical and Bioanalytical Chemistry (2021) 475 – 483, https://doi.org/10.1007/s00216-021-03288-6
- Kirschbaum-Harriman, S. E. K., Mayer, M., Duerkop, A., Hirsch, T., Baeumner, A.J. „Signal enhancement and low oxidation potential for miniaturized ECL biosensors via N-butyldiethanolamine“ (2017) Analyst, 2017, 142, 2469 – 2474, DOI: 10.1039/C7AN00261K
- Genslein, C., Hausler, P, Kirchner, E-M., Bierl, R., Baeumner, A.J., Hirsch, T. „Graphene-enhanced plasmonic nanohole arrays for environmental sensing in aqueous samples“ Beilstein J. Nanotechnol., vol. 7, pp. 1564 – 1573 (2016), DOI: 10.3762/bjnano.7.150
- Matlock-Colangelo, L.E., Coon, B., Pitner, C.L., Frey, M.W., Baeumner, A.J. “Functionalized electrospun poly(vinyl alcohol) nanofibers for on-chip concentration of E. coli cells” Analytical and Bioanalytical Chemistry, 408(5), 1327-1334 (2016), DOI: 10.1007/s00216-015-9112-5
- Fenzl, C., Genslein, C., Zoepfl, A., Baeumner, A.J., Hirsch, T. “Photonic crystal based sensing scheme for acetylchloline and acetylcholinesterase inhibitors” J. Materials Chemistry B vol. 3, pp. 2089 – 2095 (2015), DOI: 10.1039/c4tb01970a
- Reinholt, S., Behrent, A., Greene, C., Kalfe, A., Baeumner, A.J. “Isolation and Amplification of mRNA within a Simple Microfluidic Lab on a Chip” Anal. Chem vol. 86(1), pp. 849 - 856 (2014), DOI: 10.1021/ac403417z, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923508/
- Edwards, K.A., Meyers, K.J., Leonard, B., Connelly, J.T., Wang, Y., Holter, T., Baeumner, A.J. “Engineering Liposomes as Detection Reagents for CD4+ T-cells” Analytical Methods, vol. 4 (12), pp. 3948 – 3955 (2012), DOI: 10.1039/C2AY25480H
- Connelly, J.T; Kondapalli, S., Skoupi, M., Parker, S.L., Kirby, B.J, Baeumner. A.J. “Micro-total analysis system for virus detection: microfluidic pre-concentration coupled to liposome-based detection” Analytical and Bioanalytical Chemistry, 402(1) pp. 315-323 (2012) DOI: 10.1007/s00216-011-5381-9
- Nugen, S.R., Asiello, P.J., Connelly, J.T., Baeumner, A.J. “PMMA biosensor for nucleic acids with integrated mixer and electrochemical detection” Biosensors and Bioelectronics, vol. 24, pp. 2428 – 2433 (2009), DOI: 10.1016/j.bios.2008.12.025
- Bunyakul, N., Edwards, K.A., Promptmas, C., Baeumner, A.J. “Cholera toxin subunit B detection in microfluidic devices” Analytical and Bioanalytical Chemistry, vol. 393(1), p. 177 – 186, Special anniversary issue. (2009), DOI: 10.1007/s00216-008-2364-6
- Natalya V. Zaytseva, Vasiliy N. Goral, Richard A. Montagna, and Antje J. Baeumner “Development of a microfluidic biosensor module for pathogen detection” Lab-on-chip, vol 5 (8), pp. 805 – 811 (2005), DOI: 10.1039/B503856A (issue cover picture)
- Baeumner, A.J., Pretz, J., Fang, S. “A Universal Nucleic Acid Sequence Biosensor with Nanomolar Detection Limits”, Analytical Chemistry, vol. 76 (4), pp. 888 – 894 (2004), DOI: 10.1021/ac034945l