Hydrogen peroxide (HP) has a central role as second messenger and reaction product of enzymatic and metabolic activity. On the other hand, HP is a reactive oxygen species and cell-toxic. Therefore, the oxidative stress of cells may be monitored by probes for HP via the activity of oxidases. Hence, we develop new luminescent sensors and lanthanide complexes to probe HP via luminescence intensity and lifetime. Those parameters are acquired either in microtiter plate assays (for high sample throughput) but as well in sensors for the continuous measurement of HP. Latest sensor microplates just require sample addition and incubation and provide record-low limits of detection down to 5 nM HP
Selected publications:
Galligan JJ, Baeumner AJ, Duerkop A, 2025, A fluorescent MTP-based detection platform for hydrogen peroxide, glucose, and lactate, Anal Sens, e202500060, DOI:10.1002/anse.202500060 (externer Link, öffnet neues Fenster)
Galligan JJ, Baeumner AJ, Dürkop A, 2024, Recent advances and trends in optical devices and sensors for hydrogen peroxide detection, TrAC - Trends in Analytical Chemistry, 180:117948, DOI: 10.1016/j.trac.2024.117948 (externer Link, öffnet neues Fenster)
Burmistrova NA, Kolontaeva OA, Duerkop A, 2015, New Nanomaterials and Luminescent Optical Sensors for Detection of Hydrogen Peroxide, Chemosensors 3:253-273, DOI:10.3390/chemosensors3040253 (externer Link, öffnet neues Fenster)
Burmistrova NA, Meier RJ, Schreml S, Duerkop A, 2014, Reusable Optical Sensing Microplate for Hydrogen Peroxide Using a Fluorescent Photoinduced Electron Transfer Probe (HP Green), Sens Actuat B 193:799-805, DOI: 10.1016/j.snb.2013.12.025 (externer Link, öffnet neues Fenster)
Wolfbeis OS, Dürkop A, Wu M, Lin Z, 2002, Europium Ion-Based Luminescent Sensing Probe for Hydrogen Peroxide, Angew Chem Int Ed, 41:4495-4498, DOI: 10.1002/1521-3773(20021202)41:23<4495::AID-ANIE4495>3.0.CO;2- (externer Link, öffnet neues Fenster)