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• "Constitutively Active Glutaminase Variants Provide Insights into the Activation Mechanism of Anthranilate Synthase", List, Bocola, Haeger, Sterner, 2012

  Publikation

  The glutamine amidotransferase (GATase) family comprises enzyme complexes which consist of glutaminase and synthase subunits that catalyze in a...

  Publikation auf epub.uni-regensburg.de: "Constitutively Active Glutaminase Variants Provide Insights into the Activation Mechanism of Anthranilate Synthase" (externer Link, öffnet neues Fenster)

• "Directed evolution of ( )8-barrel enzymes: establishing phosphoribosylanthranilate isomerisation activity on the scaffold of the tryptophan synthase -subunit", Evran, Telefoncu, Sterner, 2012

  Publikation

  Phosphoribosylanthranilate (PRA) isomerase (TrpF) and tryptophan synthase -subunit (TrpA) are ()(8)-barrel enzymes that are involved in the...

  Publikation auf epub.uni-regensburg.de: "Directed evolution of ( )8-barrel enzymes: establishing phosphoribosylanthranilate isomerisation activity on the scaffold of the tryptophan synthase -subunit" (externer Link, öffnet neues Fenster)

• "Random mutagenesis suggests that sequence errors are not a major cause of variation in the activity of individual molecules of β-galactosidase", Craig, Schwab, Sterner, 2012

  Publikation

  Wild-type Escherichia coli lacZ was subjected to error-prone PCR to generate two plasmid-encoded gene libraries containing approximately 2.6 (SD 1.9)...

  Publikation auf epub.uni-regensburg.de: "Random mutagenesis suggests that sequence errors are not a major cause of variation in the activity of individual molecules of β-galactosidase" (externer Link, öffnet neues Fenster)

• "Folding Mechanism of an Extremely Thermostable (βα)8-Barrel Enzyme: A High Kinetic Barrier Protects the Protein from Denaturation", Carstensen, Zoldák, Schmid, Sterner, 2012

  Publikation

  HisF, the cyclase subunit of imidazole glycerol phosphate synthase (ImGPS) from Thermotoga maritima, is an extremely thermostable (beta...

  Publikation auf epub.uni-regensburg.de: "Folding Mechanism of an Extremely Thermostable (βα)8-Barrel Enzyme: A High Kinetic Barrier Protects the Protein from Denaturation" (externer Link, öffnet neues Fenster)

• "A Fast and Precise Approach for Computational Saturation Mutagenesis and its Experimental Validation by Using an Artificial (βα)8-Barrel Protein", Fischer, Seitz, Lochner, Sterner, Merkl, Bocola, 2011

  Publikation

  We present a computational saturation mutagenesis protocol (CoSM) that predicts the impact on stability of all possible amino acid substitutions for a...

  Publikation auf epub.uni-regensburg.de: "A Fast and Precise Approach for Computational Saturation Mutagenesis and its Experimental Validation by Using an Artificial (βα)8-Barrel Protein" (externer Link, öffnet neues Fenster)

• "A sugar isomerization reaction established on various ( )8-barrel scaffolds is based on substrate-assisted catalysis", Reisinger, Bocola, List, Claren, Rajendran, Sterner, 2012

  Publikation

  In the course of tryptophan biosynthesis, the isomerization of phosphoribosylanthranilate (PRA) is catalyzed by the (beta alpha)(8)-barrel enzyme...

  Publikation auf epub.uni-regensburg.de: "A sugar isomerization reaction established on various ( )8-barrel scaffolds is based on substrate-assisted catalysis" (externer Link, öffnet neues Fenster)

• "Dimerization determines substrate specificity of a bacterial prenyltransferase", Peterhoff, Zellner, Guldan, Merkl, Sterner, Babinger, 2012

  Publikation

  We have identified the native dimer interface of heptaprenylglyceryl phosphate synthase PcrB from the bacterium Bacillus subtilis and analyzed the...

  Publikation auf epub.uni-regensburg.de: "Dimerization determines substrate specificity of a bacterial prenyltransferase" (externer Link, öffnet neues Fenster)

• "Catalysis Uncoupling in a Glutamine Amidotransferase Bienzyme by Unblocking the Glutaminase Active Site", List, Vega, Razeto, Häger, Sterner, Wilmanns, 2012

  Publikation

  Nitrogen is incorporated into various metabolites by multifunctional glutamine amidotransferases via reactive ammonia generated by glutaminase...

  Publikation auf epub.uni-regensburg.de: "Catalysis Uncoupling in a Glutamine Amidotransferase Bienzyme by Unblocking the Glutaminase Active Site" (externer Link, öffnet neues Fenster)

• "Identification and Characterization of Heptaprenylglyceryl Phosphate Processing Enzymes inBacillus subtilis", Linde, Peterhoff, Sterner, Babinger, 2016

  Publikation

  In Archaea, ether lipids play an essential role as the main building blocks of the cellular membrane. Recently, ether lipids have also been discovered...

  Publikation auf epub.uni-regensburg.de: "Identification and Characterization of Heptaprenylglyceryl Phosphate Processing Enzymes inBacillus subtilis" (externer Link, öffnet neues Fenster)

• "Ancestral Tryptophan Synthase Reveals Functional Sophistication of Primordial Enzyme Complexes", Busch, Rajendran, Heyn, Schlee, Merkl, Sterner, 2016

  Publikation

  Modern enzyme complexes are characterized by a high catalytic efficiency and allosteric communication between the constituting protein subunits. We...

  Publikation auf epub.uni-regensburg.de: "Ancestral Tryptophan Synthase Reveals Functional Sophistication of Primordial Enzyme Complexes" (externer Link, öffnet neues Fenster)